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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-85643 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GM130 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Bovine (85%). Positive Control: 293T, A431, HeLa, HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.45 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Mutations in COPA lead to abnormal trafficking of STING to the Golgi and interferon signaling.
Lepelley A, Martin-Niclós MJ, Le Bihan M, Marsh JA, Uggenti C, Rice GI, Bondet V, Duffy D, Hertzog J, Rehwinkel J, Amselem S, Boulisfane-El Khalifi S, Brennan M, Carter E, Chatenoud L, Chhun S, Coulomb l'Hermine A, Depp M, Legendre M, Mackenzie KJ, Marey J, McDougall C, McKenzie KJ, Molina TJ, Neven B, Seabra L, Thumerelle C, Wislez M, Nathan N, Manel N, Crow YJ, Frémond ML
The Journal of experimental medicine 2020 Nov 2;217(11)
The Journal of experimental medicine 2020 Nov 2;217(11)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GM130 Polyclonal Antibody detects GM130 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GM130 Polyclonal Antibody (Product # PA5-85643) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GM130 in various whole cell extracts using GM130 polyclonal antibody (Product # PA5-85643) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of GM130 in 4% paraformaldehyde-fixed HeLa cells using GM130 polyclonal antibody (Product # PA5-85643) at a dilution of 1:200. Sample was then incubated with Hoechst secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of GM130 in HeLa whole cell extracts with GM130 polyclonal antibody (Product # PA5-85643) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of GM130 in HeLa whole cell extracts with GM130 polyclonal antibody (Product # PA5-85643) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
