M05865-2

antibody from Boster Biological Technology

Targeting: GOLGA2 GM130, golgin-95

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

M05865-2

Provider

Boster Biological Technology

Product name

Anti-GM130 GOLGA2 Antibody Picoband™ (monoclonal, 6D4)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for GM130 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

6D4

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

References

Submitted references

Label-free nonlinear optical signatures of extracellular vesicles in liquid and tissue biopsies of human breast cancer.

Sorrells JE, Park J, Aksamitiene E, Marjanovic M, Martin EM, Chaney EJ, Higham AM, Cradock KA, Liu ZG, Boppart SA
Scientific reports 2024 Mar 6;14(1):5528

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Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism.

Gao H, Zeng Y, Huang X, A L, Liang Q, Xie J, Lin X, Gong J, Fan X, Zou T, Xu H
Journal of extracellular vesicles 2024 Jan;13(1):e12401

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Tetramethylpyrazine ameliorates endotoxin-induced acute lung injury by relieving Golgi stress via the Nrf2/HO-1 signaling pathway.

Li S, Xu Y, He S, Li X, Shi J, Zhang B, Zhu Y, Li X, Wang Y, Liu C, Ma Y, Dong S, Yu J
BMC pulmonary medicine 2023 Aug 7;23(1):286

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of GM130 using anti- GM130 antibody (M05865-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: Hela whole cell lysates, Lane 3: K562 whole cell lysates, Lane 4: Caco-2 whole cell lysates, Lane 5: A549 whole cell lysates, Lane 6: A431 whole cell lysates, Lane 7: HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti- GM130 antigen affinity purified monoclonal antibody (Catalog # M05865-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for GM130 at approximately 130KD. The expected band size for GM130 is at 130KD.

Additional image