Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP1-89757 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89757, RRID:AB_11037808
- Product name
- Rabbit Polyclonal GM130/GOLGA2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human GM130/GOLGA2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Wdpcp, a PCP protein required for ciliogenesis, regulates directional cell migration and cell polarity by direct modulation of the actin cytoskeleton.
Cui C, Chatterjee B, Lozito TP, Zhang Z, Francis RJ, Yagi H, Swanhart LM, Sanker S, Francis D, Yu Q, San Agustin JT, Puligilla C, Chatterjee T, Tansey T, Liu X, Kelley MW, Spiliotis ET, Kwiatkowski AV, Tuan R, Pazour GJ, Hukriede NA, Lo CW
PLoS biology 2013 Nov;11(11):e1001720
PLoS biology 2013 Nov;11(11):e1001720
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis in human cell line U-138 MG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar pattern to independent antibody NBP1-89756 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GM130/GOLGA2 Antibody [NBP1-89757] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human parathyroid gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining in human parathyroid gland and lymph node tissues using anti-GOLGA2 antibody. Corresponding GOLGA2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex, colon, lymph node and testis using Anti-GOLGA2 antibody NBP1-89757 (A) shows similar protein distribution across tissues to independent antibody NBP1-89756 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human cerebral cortex.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human testis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: GM130/GOLGA2 Antibody [NBP1-89757] - Immunohistochemical staining of human lymph node shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: GM130/GOLGA2 Antibody [NBP1-89757] - Staining of human lymph node using Anti-GOLGA2 antibody NBP1-89757.