PA5-80788

antibody from Invitrogen Antibodies

Targeting: MAGOH MAGOH1, MAGOHA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-80788

Provider

Invitrogen Antibodies

Product name

MAGOH Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%). This antibody has specificity for Human MAGOH.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MAGOH in Lane A: Jurkat Whole Cell Lysate (30 µg), Lane B: Hela Whole Cell Lysate (30 µg), Lane C: A431 Whole Cell Lysate (30 µg), Lane D: K562 Whole Cell Lysate (30 µg). Samples were probed using a MAGOH Polyclonal Antibody (Product # PA5-80788) at a 1:500 dilution, followed by a Goat Anti-Rabbit IgG (H+L), Dylight 800 Secondary Antibody at a 1:10000 dilution. Western blot was performed under reducing conditions. Predicted band size:17 kDa. Observed band size:15 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MAGOH Polyclonal Antibody (Product # PA5-80788) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: Hela Whole Cell Lysate, Lane C: A431 Whole Cell Lysate, Lane D: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 15 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MAGOH Immunoprecipitation using: Lane A: 0.5 mg 293T Whole Cell Lysate 2 µL with MAGOH Polyclonal Antibody (Product # PA5-80788) and 60 μg of Immunomagnetic beads Protein G. Primary antibody: MAGOH Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 17 kDa. Observed band size: 17 kDa.