MA1-5777

antibody from Invitrogen Antibodies

Targeting: GEMIN2 SIP1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

MA1-5777

Provider

Invitrogen Antibodies

Product name

Gemin 2 Monoclonal Antibody (2E17)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-5777 detects Gemin2 in human and xenopus samples. MA1-5777 has been successfully used in Western blot, immunoprecipitation, immunofluorescence and ELISA procedures. MA1-5777 detects a band of approximately 34 kDa. The MA1-5777 immunogen is His-Gemin2 (starting at 2nd methionine). Store at 4ºC for one month, or in small aliquots at -20ºC for longer periods.

Reactivity

Human, Mouse, Xenopus

Host

Mouse

Isotype

IgG

Antibody clone number

2E17

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Gemin 2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gemin 2 Mouse Monoclonal Antibody (Product # MA1-5777) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nucleus and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.