GTX629540

antibody from GeneTex

Targeting: UBA7 D8, UBA1B, UBE1L, UBE2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX629540

Provider

GeneTex

Product name

UBA7 antibody [EPR4269(2)], N-term

Antibody type

Monoclonal

Host

Rabbit

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HA tag antibody [GT134] detects HA tag protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract B. 30 ?g whole cell lysate/extract of HA tagged protein expressing HeLa cells7.5 % SDS-PAGEHA tag antibody [GT134] (GTX629540) dilution: 1:5000

Validation comment

WB

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HA tag antibody [GT134] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 £gg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 £gg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 £gg of HA tag antibody [GT134] (GTX629540) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT134] (GTX629540) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.