Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
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Validation data
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- Product number
- ABIN497973 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-ATG7 Autophagy Related 7 Homolog (S. Cerevisiae) (ATG7) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- APG7 antibody was raised against a 17 amino acid peptide from near the carboxy terminus of Human APG7L/ATG7 (NP_006386).
- Description
- Immunoaffinity Chromatography
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Autophagy as a cell death and tumor suppressor mechanism.
Tor-mediated induction of autophagy via an Apg1 protein kinase complex.
A protein conjugation system essential for autophagy.
Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.
Gozuacik D, Kimchi A
Oncogene 2004 Apr 12;23(16):2891-906
Oncogene 2004 Apr 12;23(16):2891-906
Tor-mediated induction of autophagy via an Apg1 protein kinase complex.
Kamada Y, Funakoshi T, Shintani T, Nagano K, Ohsumi M, Ohsumi Y
The Journal of cell biology 2000 Sep 18;150(6):1507-13
The Journal of cell biology 2000 Sep 18;150(6):1507-13
A protein conjugation system essential for autophagy.
Mizushima N, Noda T, Yoshimori T, Tanaka Y, Ishii T, George MD, Klionsky DJ, Ohsumi M, Ohsumi Y
Nature 1998 Sep 24;395(6700):395-8
Nature 1998 Sep 24;395(6700):395-8
Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells.
Kisen GO, Tessitore L, Costelli P, Gordon PB, Schwarze PE, Baccino FM, Seglen PO
Carcinogenesis 1993 Dec;14(12):2501-5
Carcinogenesis 1993 Dec;14(12):2501-5
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ATG7 in Caco-2 cell lysate with ATG7 Antibody Cat.-N AP20055PU-N at (A) 0.5, (B) 1 and (C) 2 ?g/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence of ATG7 in MCF7 cells with ATG7 Antibody Cat.-N AP20055PU-N at 20 μg/ml.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of ATG7 in MCF7 cells with ATG7 Antibody Cat.-N AP20055PU-N at 10 ?g/ml.