Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 12-2229-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD319 (CRACC) Monoclonal Antibody (162), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 162 recognizes CRACC (CD2 like receptor for activating cytotoxic cells) also known as CD319 and CS1. It is a member of the SLAM receptor family. CRACC is a 66kDa glycosylated transmembrane protein which contains a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif). It is this domain, found in other proteins such as CD244 and NTB-A, that interacts with SH2 containing proteins such as SAP. Interestingly the ITSM domain in CRACC is SAP independent. Instead EAT-2 acts as the signaling molecule for CRACC. This has profound impact for patients with X-linked lymphoproliferative disease (contains a SAP mutation). It should be noted that a splice variant has been reported which deletes the ITSM domain. Applications Reported: This 162 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 162 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- 162
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family.
Bouchon A, Cella M, Grierson HL, Cohen JI, Colonna M
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5517-21
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5517-21
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0086-42) and Mouse IgG2a kappa Isotype Control PE (Product # 12-4732-81) (left) or Anti-Human CD319 (CRACC) PE (right). Cells in the lymphocyte gate were used for analysis.
- Conjugate
- Yellow dye