Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 46-2229-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD319 (CRACC) Monoclonal Antibody (162), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody 162 recognizes CRACC (CD2 like receptor for activating cytotoxic cells) also known as CD319 and CS1. It is a member of the SLAM receptor family. CRACC is a 66 kDa glycosylated transmembrane protein which contains a cytoplasmic ITSM domain (Immunoreceptor Tyrosine-based Switch Motif). It is this domain, found in other proteins such as CD244 and NTB-A, that interacts with SH2 containing proteins such as SAP. Interestingly the ITSM domain in CRACC is SAP independent. Instead EAT-2 acts as the signaling molecule for CRACC. This has profound impact for patients with X-linked lymphoproliferative disease (contains a SAP mutation). It should be noted that a splice variant has been reported which deletes the ITSM domain. Applications Reported: This 162 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 162 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 162
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Activation of NK cell-mediated cytotoxicity by a SAP-independent receptor of the CD2 family.
Bouchon A, Cella M, Grierson HL, Cohen JI, Colonna M
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5517-21
Journal of immunology (Baltimore, Md. : 1950) 2001 Nov 15;167(10):5517-21
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD8a APC (Product # 17-0088-42) and Mouse IgG2b K Isotype Control PerCP-eFluor® 710 (Product # 46-4732-82) (left) or Anti-Human CD319 (CRACC) PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.