19936-1-AP

antibody from Proteintech Group

Targeting: CYP1A2 CP12, P3-450

Provider product page for 19936-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [35]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 19936-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#19936-1-AP, RRID:AB_10638909
Product name: CYP1A2-Specific antibody
Antibody type: Polyclonal
Description: CYP1A2-Specific antibody (Cat. #19936-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CYP1A2 protein structure - 19936-1-AP shown in red.

Submitted references Proteome characterization of liver-kidney comorbidity after microbial sepsis.

Gui Y, Yu Y, Wang W, Wang Y, Lu H, Mozdzierz S, Eskander K, Lin YH, Li H, Tian XJ, Liu S, Zhou D
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2024 Apr 15;38(7):e23597

Microneedle array facilitates hepatic sinusoid construction in a large-scale liver-acinus-chip microsystem.

Li S, Li C, Khan MI, Liu J, Shi Z, Gao D, Qiu B, Ding W
Microsystems & nanoengineering 2023;9:75

Eight Zhes Decoction ameliorates the lipid dysfunction of nonalcoholic fatty liver disease using integrated lipidomics, network pharmacology and pharmacokinetics.

Zhou Y, Dai Z, Deng K, Wang Y, Ying J, Chu D, Zhou J, Tang C
Journal of pharmaceutical analysis 2023 Sep;13(9):1058-1069

ABCB11 accumulated in immature tertiary lymphoid structures participates in xenobiotic metabolic process and predicts resistance to PD-1/PD-L1 inhibitors in head and neck squamous cell carcinoma.

Ning J, Hao J, Guo F, Hou X, Li L, Wang J, Wang S, Gao Y, Zheng X, Gao M
Translational oncology 2023 Oct;36:101747

Modeling Colorectal Cancer-Induced Liver Portal Vein Microthrombus on a Hepatic Lobule Chip.

Du X, Zou R, Du K, Huang D, Miao C, Qiu B, Ding W, Li C
ACS applied materials & interfaces 2023 Nov 30;

Impact of tectoridin on the pharmacokinetics of florfenicol via targeting cytochrome P450 and P-glycoprotein of rats.

Zhang M, Wang B, Li XT, Yin Q, Liang G, Li SC
Xenobiotica; the fate of foreign compounds in biological systems 2023 May;53(5):429-437

Liberation of daidzein by gut microbial β-galactosidase suppresses acetaminophen-induced hepatotoxicity in mice.

Zeng Y, Wu R, Wang F, Li S, Li L, Li Y, Qin P, Wei M, Yang J, Wu J, Chen A, Ke G, Yan Z, Yang H, Chen Z, Wang Z, Xiao W, Jiang Y, Chen X, Zeng Z, Zhao X, Chen P, Gong S
Cell host & microbe 2023 May 10;31(5):766-780.e7

Coptisine modulates the pharmacokinetics of florfenicol by targeting CYP1A2, CYP2C11 and CYP3A1 in the liver and P-gp in the jejunum of rats: a pilot study.

Li SC, Zhang M, Wang B, Li XT, Liang G
Xenobiotica; the fate of foreign compounds in biological systems 2023 Mar;53(3):207-214

Integration of proteomics and network toxicology reveals the mechanism of mercury chloride induced hepatotoxicity, in mice and HepG2 cells.

Cao X, Mao K, Zhang Y, Yang M, Liu H, Wang X, Hao L
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association 2023 Jul;177:113820

Glutaredoxin-1 alleviates acetaminophen-induced liver injury by decreasing its toxic metabolites.

Xu Y, Xia Y, Liu Q, Jing X, Tang Q, Zhang J, Jia Q, Zhang Z, Li J, Chen J, Xiong Y, Li Y, He J
Journal of pharmaceutical analysis 2023 Dec;13(12):1548-1561

Air-ventilated normothermic machine perfusion alleviates hepatic injury from DCD rat through CYP1A2.

Shi JH, Cheng N, Zhang W, Jin Q, Guo WZ, Ge GB, Zhang SJ, Line PD, Wang Y
Heliyon 2023 Aug;9(8):e19150

Intermittent Hypoxia Inhibits Hepatic CYP1a2 Expression and Delays Aminophylline Metabolism.

Zhang XB, Chen XY, Chiu KY, He XZ, Wang JM, Zeng HQ, Zeng Y
Evidence-based complementary and alternative medicine : eCAM 2022;2022:2782702

Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism.

Uehara S, Iida Y, Ida-Tanaka M, Goto M, Kawai K, Yamamoto M, Higuchi Y, Ito S, Takahashi R, Kamimura H, Ito M, Yamazaki H, Oshimura M, Kazuki Y, Suemizu H
Scientific reports 2022 Sep 1;12(1):14907

Gut microbiota and host Cyp450s co-contribute to pharmacokinetic variability in mice with non-alcoholic steatohepatitis: Effects vary from drug to drug.

Guo J, Xu Y, Chen LJ, Zhang SX, Liou YL, Chen XP, Tan ZR, Zhou HH, Zhang W, Chen Y
Journal of advanced research 2022 Jul;39:319-332

Kinetic Characteristics of Curcumin and Germacrone in Rat and Human Liver Microsomes: Involvement of CYP Enzymes.

Su S, Wu H, Zhou J, Yuan G, Wang H, Feng J
Molecules (Basel, Switzerland) 2022 Jul 14;27(14)

Is platelet responsiveness to clopidogrel attenuated in overweight or obese patients and why? A reverse translational study in mice.

Jiang LP, Ji JZ, Ge PX, Zhu T, Mi QY, Tai T, Li YF, Xie HG
British journal of pharmacology 2022 Jan;179(1):46-64

Proteomic and Bioinformatics Analysis of Membrane Lipid Domains after Brij 98 Solubilization of Uninduced and Phenobarbital-Induced Rat Liver Microsomes: Defining the Membrane Localization of the P450 Enzyme System.

Reed JR, Guidry JJ, Backes WL
Drug metabolism and disposition: the biological fate of chemicals 2022 Apr;50(4):374-385

Aryl Hydrocarbon Receptor Activity in Hepatocytes Sensitizes to Hyperacute Acetaminophen-Induced Hepatotoxicity in Mice.

Schuran FA, Lommetz C, Steudter A, Ghallab A, Wieschendorf B, Schwinge D, Zuehlke S, Reinders J, Heeren J, Lohse AW, Schramm C, Herkel J, Carambia A
Cellular and molecular gastroenterology and hepatology 2021;11(2):371-388

Modeling nonalcoholic fatty liver disease on a liver lobule chip with dual blood supply.

Du K, Li S, Li C, Li P, Miao C, Luo T, Qiu B, Ding W
Acta biomaterialia 2021 Oct 15;134:228-239

Macrophage potentiates the recovery of liver zonation and metabolic function after acute liver injury.

Miura A, Hosono T, Seki T
Scientific reports 2021 May 6;11(1):9730

Identification of the contact region responsible for the formation of the homomeric CYP1A2•CYP1A2 complex.

Saha A, Connick JP, Reed JR, Lott CS, Backes WL
The Biochemical journal 2021 Jun 11;478(11):2163-2178

AHR mediates the aflatoxin B1 toxicity associated with hepatocellular carcinoma.

Zhu Q, Ma Y, Liang J, Wei Z, Li M, Zhang Y, Liu M, He H, Qu C, Cai J, Wang X, Zeng Y, Jiao Y
Signal transduction and targeted therapy 2021 Aug 9;6(1):299

Short-term standard alcohol consumption enhances platelet response to clopidogrel through inhibition of Nrf2/Ces1 pathway and induction of Cyp2c in mice.

Ge PX, Jiang LP, Tai T, Zhu T, Ji JZ, Li YF, Mi QY, Xie HG
Life sciences 2021 Aug 15;279:119268

Effects of Saikosaponin D on CYP1A2 and CYP2D6 in HepaRG Cells.

Li H, Tang Y, Wang Y, Wei W, Yin C, Tang F
Drug design, development and therapy 2020;14:5251-5258

Sensing the Generation of Intracellular Free Electrons Using the Inactive Catalytic Subunit of Cytochrome P450s as a Sink.

Akintade DD, Chaudhuri B
Sensors (Basel, Switzerland) 2020 Jul 21;20(14)

Knockdown of Long Noncoding RNAs Hepatocyte Nuclear Factor 1α Antisense RNA 1 and Hepatocyte Nuclear Factor 4α Antisense RNA 1 Alters Susceptibility of Acetaminophen-Induced Cytotoxicity in HepaRG Cells.

Chen L, Wang P, Manautou JE, Zhong XB
Molecular pharmacology 2020 Apr;97(4):278-286

CYP enzymes, expressed within live human suspension cells, are superior to widely-used microsomal enzymes in identifying potent CYP1A1/CYP1B1 inhibitors: Identification of quinazolinones as CYP1A1/CYP1B1 inhibitors that efficiently reverse B[a]P toxicity and cisplatin resistance.

Sonawane VR, Siddique MUM, Gatchie L, Williams IS, Bharate SB, Jayaprakash V, Sinha BN, Chaudhuri B
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2019 Apr 1;131:177-194

Schisantherin A alleviated alcohol-induced liver injury by the regulation of alcohol metabolism and NF-kB pathway.

Li B, Li D, Wang Y, Meng X, Sun X, Tian J, Shi L, Ma F
Experimental animals 2018 Nov 1;67(4):451-461

Drug resistance induces the upregulation of H(2)S-producing enzymes in HCT116 colon cancer cells.

Untereiner AA, Pavlidou A, Druzhyna N, Papapetropoulos A, Hellmich MR, Szabo C
Biochemical pharmacology 2018 Mar;149:174-185

Poly(ADP-ribosyl)ated PXR is a critical regulator of acetaminophen-induced hepatotoxicity.

Wang C, Xu W, Zhang Y, Huang D, Huang K
Cell death & disease 2018 Jul 26;9(8):819

Decreased expression of hepatic cytochrome P450 1A2 (CYP1A2) in a chronic intermittent hypoxia mouse model.

Zhang XB, Zeng YM, Chen XY, Zhang YX, Ding JZ, Xue C
Journal of thoracic disease 2018 Feb;10(2):825-834

Hepatic, Metabolic, and Toxicity Evaluation of Repeated Oral Administration of SnS2 Nanoflowers in Mice.

Bai D, Li Q, Xiong Y, Wang C, Shen P, Bai L, Yuan L, Wu P
Toxicological sciences : an official journal of the Society of Toxicology 2018 Aug 1;164(2):501-511

Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues.

Gao Y, Wang X, Sang Z, Li Z, Liu F, Mao J, Yan D, Zhao Y, Wang H, Li P, Ying X, Zhang X, He K, Wang H
Scientific reports 2017 Apr 5;7:45913

Self-renewal of hepatoblasts under chemically defined conditions by iterative growth factor and chemical screening.

Lv L, Han Q, Chu Y, Zhang M, Sun L, Wei W, Jin C, Li W
Hepatology (Baltimore, Md.) 2015 Jan;61(1):337-47

Different expression patterns of hepatic cytochrome P450 s during anaphylactic or lipopolysaccharide-induced inflammation.

Moriya N, Kataoka H, Fujino H, Nishikawa J, Kugawa F
Die Pharmazie 2014 Feb;69(2):142-7

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: mouse liver tissue were subjected to SDS PAGE followed by western blot with 19936-1-AP(CYP1A2-Specific antibody) at dilution of 1:1000
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using CYP1A2 antibody 19936-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line