TA505958

antibody from OriGene

Targeting: CTAG1B CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, NY-ESO-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

TA505958

Provider

OriGene

Proper citation

OriGene Cat#TA505958, RRID:AB_2623500

Product name

Purified CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI5A5 (formerly 5A5)

Antibody type

Monoclonal

Description

Purified CTAG1B (NY-ESO-1) mouse monoclonal antibody, clone OTI5A5 (formerly 5A5)

Host

Mouse

Conjugate

Unconjugated

Epitope

CTAG1B

Isotype

IgG

Antibody clone number

OTI5A5

Vial size

100 µl

Concentration

1 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (RC213318, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-CTAG1B monoclonal antibody at 1:200.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-CTAG1B mouse monoclonal antibody (TA505958) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CTAG1B(RC213318).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505958)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505958)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505958)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505958)

Validation comment

IHC