NBP1-47714
antibody from Novus Biologicals
		Targeting: CTAG1B
		
		CT6.1, CTAG, CTAG1, ESO1, LAGE2A, LAGE2B, NY-ESO-1	
	
	
	
	
Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [2]
 - Immunohistochemistry [9]
 - Flow cytometry [3]
 
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Validation data
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- Product number
 - NBP1-47714 - Provider product page

 - Provider
 - Novus Biologicals
 - Proper citation
 - Novus Cat#NBP1-47714, RRID:AB_10010680
 - Product name
 - Mouse Monoclonal CTAG1B Antibody
 - Antibody type
 - Monoclonal
 - Description
 - Affinity purified.
 - Reactivity
 - Human
 - Host
 - Mouse
 - Isotype
 - IgG
 - Vial size
 - 0.1 ml
 - Concentration
 - 1 mg/ml
 - Storage
 - Store at -20C. Avoid freeze-thaw cycles.
 
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					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CTAG1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CTAG1B.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Western blot analysis of extracts (35ug) from 2 cell lines lysates by using anti-CTAG1B monoclonal antibody (1:500).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of kidney tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of lung tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of pancreas tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of prostate tissue using anti-CTAG1B mouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-CTAG1Bmouse monoclonal antibody.
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunohistochemistry-Paraffin: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-CTAG1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100 degrees C for 10min, NBP1-47714, Dilution 1:50)
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living 293T cells transfected with CTAG1B overexpression plasmid (RC213318), Red)/empty vector (Blue) using anti-CTAG1B antibody. Cells incubated with a non-specific antibody (Green) were used as isotype control. (1:100)
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living NCI-H1299 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)
 
- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Flow Cytometry: CTAG1B Antibody (OTI2B6) [NBP1-47714] - Analysis of living A375 cells, using anti-CTAG1B antibody(red), compared to an isotype control (green).(1:100)