PA1-32094

antibody from Invitrogen Antibodies

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Antibody data

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Validation data

Product number: PA1-32094 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-KPNA2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX26036-PEP).. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

KPNA2 protein structure - PA1-32094 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of KPNA2 in Hela lysate (RIPA buffer, 35 µg total protein per lane) using a KPNA2 polyclonal antibody (Product # PA1-32094) at a dilution of 0.1 µg/mL following incubation for 1 hour and detected using chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of KPNA2 was achieved by transfecting HEK-293 cells with KPNA2 specific siRNAs (Silencer® select Product # s7921, s7920). Western blot analysis (Fig. a) was performed using whole cell extracts from the KPNA2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed using KPNA2 Polyclonal Antibody (Product # PA1-32094) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KPNA2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Importin subunit alpha-1 (KPNA2) Polyclonal Antibody (Product # PA1-32094) on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), A549 (Lane 2), HeLa (Lane 3), NIH/3T3 (Lane 4), PC-12 (Lane 5), LNCaP (Lane 6), Mouse Testis (Lane 7), Mouse Thymus (Lane 8), Mouse colon (Lane 9), Mouse Spleen (Lane 10) and Rat Testis (Lane 11). A 57.86 kDa band corresponding to Importin subunit alpha-1 (KPNA2) was observed except in Mouse colon which is reported to be negative. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of KPNA2 was performed by loading 35 µg (in RIPA buffer) of HeLa lysates. Proteins were transferred to a membrane and probed with a KPNA2 Polyclonal Antibody (Product # PA1-32094) at a dilution of 0.03 µg/mL.