10819-1-AP

antibody from Proteintech Group

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

10819-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#10819-1-AP, RRID:AB_2265526

Product name

KPNA2 antibody

Antibody type

Polyclonal

Description

KD/KO validated KPNA2 antibody (Cat. #10819-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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Brennan A, Vance KW, Mason JM
Cell chemical biology 2026 Mar 19;33(3):409-423.e7

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LAMP5 modulates IRF4 stability and nuclear transport: a critical mechanism in myeloma progression and therapy.

Li Z, Liu R, Fang Z, Chen R, Yang D, Li Y, Liu S, Wang C, Liu H
Oncogene 2025 Oct;44(38):3553-3567

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The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.

Woo TT, Takeo Y, Harwood MC, Houck ET, DiMaio D, Tsai B
Science advances 2025 Oct 24;11(43):eadz6792

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The deubiquitination enzyme USP14 promotes the tumourigenesis of gastric cancer by enhancing c-MYC nuclear translocation through deubiquitination of KPNA2.

Li J, Tang H, Chang H, Du Y, Du L, Zhao E
Cell death & disease 2025 Oct 21;16(1):737

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KPNA2 silencing sensitizes triple-negative breast cancer to chemotherapy by promoting multipolar division and suppressing DNA damage repair.

Cai Y, Du J, Wang H, Shen L, Xu Z, Zhaoxiong Y, Gong Z, Zhu Y, Wu C, Cai J, Zhang P, Chen S, Dai Z, Huang R, Zhu W
Oncogene 2025 Nov;44(44):4254-4270

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piRNA28846 has the potential to be a novel RNA nucleic acid drug for ovarian cancer.

Qin HL, Han Y, Li JQ, Wu QH, Du YP, Li QH, Chen X, Wang YP, Guan X, Sheng XJ, Chen S, Zhao Y
NPJ precision oncology 2025 Mar 8;9(1):65

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Oncometabolite fumarate facilitates PD-L1 expression and immune evasion in clear cell renal cell carcinoma.

Gao Y, Fan S, Sun X, Li J, Dai Y, Li H, Ma H, Xu Y, Lv L
Cell death & disease 2025 Jun 3;16(1):432

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Elevated miR-221-3p inhibits epithelial-mesenchymal transition and biochemical recurrence of prostate cancer via targeting KPNA2: an evidence-based and knowledge-guided strategy.

Li D, Jian J, Shi M, Chen Z, Zhao A, Wei X, Huang Y, Chen Y, Hou J, Lin Y
BMC cancer 2025 Jan 8;25(1):34

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A positive feedback loop between KPNA2 and FOXM1 promotes the proliferation of lung adenocarcinoma.

Wang Y, Jin Y, Li X
European journal of medical research 2025 Dec 18;31(1):122

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KPNA2 promotes lung adenocarcinoma progression by inducing glycolysis-assisted MYC nuclear translocation.

Feng Q, Song Q, Han B, Deng Z, Yu R, Liang J
Cytotechnology 2025 Aug;77(4):158

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Machine learning-based proteomics profiling of ALS identifies downregulation of RPS29 that maintains protein homeostasis and STMN2 level.

Xu W, Guo Z, Guan Y, Lv S, Gao X, Luo W, Cheng T, Shao Z, Tao B, Wang T, Qiu Z
Communications biology 2025 Aug 7;8(1):1177

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Histone H2B lysine lactylation modulates the NF-κB response via KPNA2 during CSFV infection.

Zhu W, Zeng S, Zhu S, Zhang Z, Zhao R, Qiu Q, Luo Z, Qin Y, Chen W, Li B, He Y, Yi L, Ding H, Zhao M, Chen J, Fu C, Fan S
International journal of biological macromolecules 2025 Apr;299:139973

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Mutant p53 upregulates HDAC6 to resist ER stress and facilitates Ku70 deacetylation, which prevents its degradation and mitigates DNA damage in colon cancer cells.

Benedetti R, Di Crosta M, Gilardini Montani MS, D'Orazi G, Cirone M
Cell death discovery 2025 Apr 10;11(1):162

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Silencing of tropomodulin 1 inhibits acute myeloid leukemia cell proliferation and tumor growth by elevating karyopherin alpha 2-mediated autophagy.

Xia Y, Wang D, Zhao H, Meng T, Jiang Q, Pan Z, Wang G, An T, Li B, Bi S, Wang H, Lu J, Liu H, Lin H, Lin C, Zheng Q, Li D
Pharmacological research 2024 Sep;207:107327

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Identification of Key Immune and Cell Cycle Modules and Prognostic Genes for Glioma Patients through Transcriptome Analysis.

Guo K, Yang J, Jiang R, Ren X, Liu P, Wang W, Zhou S, Wang X, Ma L, Hu Y
Pharmaceuticals (Basel, Switzerland) 2024 Sep 28;17(10)

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Exploration and validation of ceRNA regulatory networks in colorectal cancer based on associations whole transcriptome sequencing.

Zhang L, Li Y, Fu C, Yang L, Li G, Wu Y, Tong H, Tian G, Wang K, Wang J, Ying X, Li Z
Scientific reports 2024 Sep 2;14(1):20446

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Ivermectin ameliorates acute myocarditis via the inhibition of importin-mediated nuclear translocation of NF-κB/p65.

Gao X, Xuan Y, Zhou Z, Chen C, Wen Wang D, Wen Z
International immunopharmacology 2024 May 30;133:112073

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SARS-CoV-2 Orf6 is positioned in the nuclear pore complex by Rae1 to inhibit nucleocytoplasmic transport.

Makio T, Zhang K, Love N, Mast FD, Liu X, Elaish M, Hobman T, Aitchison JD, Fontoura BMA, Wozniak RW
Molecular biology of the cell 2024 May 1;35(5):ar62

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Nuclear export of circular RNA.

Ngo LH, Bert AG, Dredge BK, Williams T, Murphy V, Li W, Hamilton WB, Carey KT, Toubia J, Pillman KA, Liu D, Desogus J, Chao JA, Deans AJ, Goodall GJ, Wickramasinghe VO
Nature 2024 Mar;627(8002):212-220

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The EGR1/miR-139/NRF2 axis orchestrates radiosensitivity of non-small-cell lung cancer via ferroptosis.

Zhang L, Xu Y, Cheng Z, Zhao J, Wang M, Sun Y, Mi Z, Yuan Z, Wu Z
Cancer letters 2024 Jul 28;595:217000

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KPNA2 promotes the progression of gastric cancer by regulating the alternative splicing of related genes.

Chen X, Wei H, Yue A, Zhang H, Zheng Y, Sun W, Zhou Y, Wang Y
Scientific reports 2024 Jul 25;14(1):17140

---

Porcine deltacoronavirus nucleocapsid protein antagonizes JAK-STAT signaling pathway by targeting STAT1 through KPNA2 degradation.

Hu Y, Hao C, Wang D, Guo M, Chu H, Jin X, Zu S, Ding X, Zhang H, Hu H
Journal of virology 2024 Jul 23;98(7):e0033424

---

DNA damage response-related signatures characterize the immune landscape and predict the prognosis of HCC via integrating single-cell and bulk RNA-sequencing.

Li H, Shi Y, Li Y, Wu S, Yang R, Liu Q, Sun L
International immunopharmacology 2024 Aug 20;137:112475

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TuBG1 promotes hepatocellular carcinoma via ATR/P53-apoptosis and cycling pathways.

Zhang Y, Wang ZZ, Han AQ, Yang MY, Zhu LX, Pan FM, Wang Y
Hepatobiliary & pancreatic diseases international : HBPD INT 2024 Apr;23(2):195-209

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Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer.

Zhang E, Ding C, Li S, Aikemu B, Zhou X, Fan X, Sun J, Yang X, Zheng M
Cancer cell international 2023 May 18;23(1):96

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Acetylation regulates the nucleocytoplasmic distribution and oncogenic function of karyopherin alpha 2 in lung adenocarcinoma.

Feng HP, Liu YC, Wang CL, Liao WC, Yu JS, Yu CJ
Biochemical and biophysical research communications 2023 Jun 4;659:96-104

---

ZNF143 facilitates the growth and migration of glioma cells by regulating KPNA2-mediated Hippo signalling.

Chen Y, Li J, Ma J, Bao Y
Scientific reports 2023 Jul 9;13(1):11097

---

ISG15 and ISGylation modulates cancer stem cell-like characteristics in promoting tumor growth of anaplastic thyroid carcinoma.

Xu T, Zhu C, Chen J, Song F, Ren X, Wang S, Yi X, Zhang Y, Zhang W, Hu Q, Qin H, Liu Y, Zhang S, Tan Z, Pan Z, Huang P, Ge M
Journal of experimental & clinical cancer research : CR 2023 Jul 27;42(1):182

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A program of successive gene expression in mouse one-cell embryos.

Asami M, Lam BYH, Hoffmann M, Suzuki T, Lu X, Yoshida N, Ma MK, Rainbow K, Gužvić M, VerMilyea MD, Yeo GSH, Klein CA, Perry ACF
Cell reports 2023 Feb 28;42(2):112023

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KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65 and IRF3: A Possible Therapeutic Approach for Atherosclerosis.

Xing Z, Zhen Y, Chen J, Du M, Li D, Liu R, Zheng J
Inflammation 2023 Dec;46(6):2071-2088

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EGFR promotes ALKBH5 nuclear retention to attenuate N6-methyladenosine and protect against ferroptosis in glioblastoma.

Lv D, Zhong C, Dixit D, Yang K, Wu Q, Godugu B, Prager BC, Zhao G, Wang X, Xie Q, Bao S, He C, Heiland DH, Rosenfeld MG, Rich JN
Molecular cell 2023 Dec 7;83(23):4334-4351.e7

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Loss of function of the ALS-associated NEK1 kinase disrupts microtubule homeostasis and nuclear import.

Mann JR, McKenna ED, Mawrie D, Papakis V, Alessandrini F, Anderson EN, Mayers R, Ball HE, Kaspi E, Lubinski K, Baron DM, Tellez L, Landers JE, Pandey UB, Kiskinis E
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Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development.

Liao W, Shi Z, Tang H, Wu T, Zhang C, He Y, Zou R, Wang L
Journal of oncology 2022;2022:6851026

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Overexpression of Karyopherin α2 in small cell carcinoma of the cervix correlates with poor prognosis.

Liao LM, Gu ZB, Fang M, Yao GJ, Huang L
International journal of clinical and experimental pathology 2022;15(6):241-246

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CREB3L1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by remodeling the tumor microenvironment.

Pan Z, Xu T, Bao L, Hu X, Jin T, Chen J, Chen J, Qian Y, Lu X, Li L, Zheng G, Zhang Y, Zou X, Song F, Zheng C, Jiang L, Wang J, Tan Z, Huang P, Ge M
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lncRNA THAP7-AS1, transcriptionally activated by SP1 and post-transcriptionally stabilized by METTL3-mediated m6A modification, exerts oncogenic properties by improving CUL4B entry into the nucleus.

Liu HT, Zou YX, Zhu WJ, Sen-Liu, Zhang GH, Ma RR, Guo XY, Gao P
Cell death and differentiation 2022 Mar;29(3):627-641

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Nuclear accumulation of KPNA2 impacts radioresistance through positive regulation of the PLSCR1-STAT1 loop in lung adenocarcinoma.

Liao WC, Lin TJ, Liu YC, Wei YS, Chen GY, Feng HP, Chang YF, Chang HT, Wang CL, Chi HC, Wang CI, Lin KH, Ou Yang WT, Yu CJ
Cancer science 2022 Jan;113(1):205-220

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BBOX1-AS1 Accelerates Nasopharyngeal Carcinoma Progression by Sponging miR-3940-3p and Enhancing KPNA2 Upregulation.

Jiang H, He Q, Liu T
Cancer management and research 2021;13:9049-9062

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Neddylation of PTEN regulates its nuclear import and promotes tumor development.

Xie P, Peng Z, Chen Y, Li H, Du M, Tan Y, Zhang X, Lu Z, Cui CP, Liu CH, He F, Zhang L
Cell research 2021 Mar;31(3):291-311

---

Centromere Protein A (CENPA) Regulates Metabolic Reprogramming in the Colon Cancer Cells by Transcriptionally Activating Karyopherin Subunit Alpha 2 (KPNA2).

Liang YC, Su Q, Liu YJ, Xiao H, Yin HZ
The American journal of pathology 2021 Dec;191(12):2117-2132

---

African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway.

Zhuo Y, Guo Z, Ba T, Zhang C, He L, Zeng C, Dai H
Virologica Sinica 2021 Apr;36(2):176-186

---

Nucleoporin 107 Promotes the Survival of Tumor Cells in Cervical Cancers.

Shi R, Xu L, Huang L, Cheng JX
Gynecologic and obstetric investigation 2020;85(1):41-52

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Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.

Fu F, Zhang Y, Gao Z, Zhao Y, Wen Z, Han H, Li Y, Chen H
International journal of cancer 2020 Jul 15;147(2):584-592

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Karyopherin α 2 promotes proliferation, migration and invasion through activating NF-κB/p65 signaling pathways in melanoma cells.

Yang F, Li S, Cheng Y, Li J, Han X
Life sciences 2020 Jul 1;252:117611

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Nuclear CD44 Mediated by Importin β Participated in Naïve Genes Transcriptional Regulation in C3A-iCSCs.

Su M, Wang P, Wang X, Zhang M, Wei S, Liu K, Han S, Han X, Deng Y, Shen L
International journal of biological sciences 2019;15(6):1252-1260

---

USP1 inhibition destabilizes KPNA2 and suppresses breast cancer metastasis.

Ma A, Tang M, Zhang L, Wang B, Yang Z, Liu Y, Xu G, Wu L, Jing T, Xu X, Yang S, Liu Y
Oncogene 2019 Mar;38(13):2405-2419

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Antiviral Immune Response as a Trigger of FUS Proteinopathy in Amyotrophic Lateral Sclerosis.

Shelkovnikova TA, An H, Skelt L, Tregoning JS, Humphreys IR, Buchman VL
Cell reports 2019 Dec 24;29(13):4496-4508.e4

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Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

Tsai CY, Chi HC, Chi LM, Yang HY, Tsai MM, Lee KF, Huang HW, Chou LF, Cheng AJ, Yang CW, Wang CS, Lin KH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May;32(5):2601-2614

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Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin-1β-induced matrix metalloproteinase expression in oral cancer.

Wang CI, Yu CJ, Huang Y, Yi JS, Cheng HW, Kao HK, Lao WW, Chang KP
Head & neck 2018 Aug;40(8):1719-1733

---

Karyopherin alpha 2 expression is a novel diagnostic and prognostic factor for colorectal cancer.

Yu L, Wang G, Zhang Q, Gao L, Huang R, Chen Y, Tang Q, Liu J, Liu C, Wang H, Wang X
Oncology letters 2017 Mar;13(3):1194-1200

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Nuclear import of Nkx2-2 is mediated by multiple pathways.

Lin W, Xu P, Guo Y, Jia Q, Tao T
Biochemical and biophysical research communications 2017 Jan 22;482(4):1511-1516

---

mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances.

Chen X, Zhu Y, Wang Z, Zhu H, Pan Q, Su S, Dong Y, Li L, Zhang H, Wu L, Lou X, Liu S
Journal of proteomics 2016 Mar 16;136:13-24

---

Quantitative proteomics reveals a novel role of karyopherin alpha 2 in cell migration through the regulation of vimentin-pErk protein complex levels in lung cancer.

Wang CI, Wang CL, Wu YC, Feng HP, Liu PJ, Chang YS, Yu JS, Yu CJ
Journal of proteome research 2015 Apr 3;14(4):1739-51

---

Quantitative proteomics reveals regulation of karyopherin subunit alpha-2 (KPNA2) and its potential novel cargo proteins in nonsmall cell lung cancer.

Wang CI, Chien KY, Wang CL, Liu HP, Cheng CC, Chang YS, Yu JS, Yu CJ
Molecular & cellular proteomics : MCP 2012 Nov;11(11):1105-22

---

Importin subunit alpha-2 is identified as a potential biomarker for non-small cell lung cancer by integration of the cancer cell secretome and tissue transcriptome.

Wang CI, Wang CL, Wang CW, Chen CD, Wu CC, Liang Y, Tsai YH, Chang YS, Yu JS, Yu CJ
International journal of cancer 2011 May 15;128(10):2364-72

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Importin-α1 as a novel prognostic target for hepatocellular carcinoma.

Yoshitake K, Tanaka S, Mogushi K, Aihara A, Murakata A, Matsumura S, Mitsunori Y, Yasen M, Ban D, Noguchi N, Irie T, Kudo A, Nakamura N, Tanaka H, Arii S
Annals of surgical oncology 2011 Jul;18(7):2093-103

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 10819-1-AP(KPNA2 antibody) at dilution of 1:1000

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human colon cancer using 10819-1-AP(KPNA2 antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The KPNA2 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human KPNA2. This antibody recognizes human,mouse,rat antigen. The KPNA2 antibody has been validated for the following applications: ELISA, WB, IHC analysis.

Appendix

10819-1-AP.pdf

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