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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB6558 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6558, RRID:AB_1576719
- Product name
- KPNA2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of KPNA2.
- Antigen sequence
C-QVQDGAPGTFNF- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Rch1, a protein that specifically interacts with the RAG-1 recombination-activating protein.
Cuomo CA, Kirch SA, Gyuris J, Brent R, Oettinger MA
Proceedings of the National Academy of Sciences of the United States of America 1994 Jun 21;91(13):6156-60
Proceedings of the National Academy of Sciences of the United States of America 1994 Jun 21;91(13):6156-60
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KPNA2 polyclonal antibody (Cat # PAB6558) staining (0.1 ug/mL) of HeLa lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- KPNA2 polyclonal antibody (Cat # PAB6558) staining (top panel and green) of 4% formaldahyde-fixed HeLa with endogenously expressed HistonH2B-GFP fusion as DNA marker (mid panel and red) . Data kindly provided by Francisco Iborra, MRC Mol. Haematol. Unit, Oxford.
- Validation comment
- Immunofluorescence
