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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- A300-484A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RCH1/KPNA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse RCH1/KPNA2 by western blot. Samples: Whole cell lysate (10 µg) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody (Product # A300-484A; lot A300-484A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCH1/KPNA2 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 antibody (Product # A300-484A; lot A300-484A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody A300-484A (lot A300-484A-2) used for IP at 6 µg per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot A300-484A-1) and rabbit anti-RCH1/KPNA2 antibody A300-483A-2. For blotting immunoprecipitated RCH1/KPNA2, A300-484A was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse RCH1/KPNA2 by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg), 293T (50µg), and mouse NIH3T3 (50µg) cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody A300-484A (lot A300-484A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse RCH1/KPNA2 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 (Product # A300-484A; lot 3) used at 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCH1/KPNA2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-RCH1/KPNA2 (Product # A300-484A lot 3) used at 1:5,000 (0.2 µg/mL). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCH1 by immunohistochemistry. Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- RCH1 (Cat. No. A300-484A Lot2) used at a dilution of 1:1,000 (1µg/ml) and 1:5,000 (0.2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody (Product # A300-484A; lot A300-484A-3) used for IP at 6 µg per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot A300-484A-2). For blotting immunoprecipitated RCH1/KPNA2 (Product # A300-484A) was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
