NB100-79808

antibody from Novus Biologicals

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-79808

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-79808, RRID:AB_1109146

Product name

Rabbit Polyclonal Importin alpha 2/KPNA2 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of Human and Mouse RCH1/KPNA2 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 ug), 293T (50ug), and mouse NIH3T3 (50ug) cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (50 ug) cells. Antibody used at 0.1 ug/ml.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Detection of human RCH1/KPNA2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-RCH1/KPNA2 antibody NB100-79808 (lot NB100-79808-2) used for IP at 6 ug per reaction. RCH1/KPNA2 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79808-1) and rabbit anti-RCH1/KPNA2 antibody NB100-79807. For blotting immunoprecipitated RCH1/KPNA2, NB100-79808 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79808] - Samples: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml) and 1:5,000 (0.2ug/ml). Detection: DAB