PA5-18237

antibody from Invitrogen Antibodies

Targeting: KPNA2 IPOA1, QIP2, RCH1, SRP1alpha

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-18237 - Provider product page
Provider: Invitrogen Antibodies
Product name: KPNA2 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody is predicted to react with canine, mouse and rat based on sequence homology.
Reactivity: Human
Host: Goat
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

KPNA2 protein structure - PA5-18237 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot staining of HeLa cell lysate using Product # PA5-18237 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of KPNA2 was achieved by transfecting HEK-293 cells with KPNA2 specific siRNAs (Silencer® select Product # s7921, s7920). Western blot analysis (Fig. a) was performed using whole cell extracts from the KPNA2 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed using KPNA2 Polyclonal Antibody (Product # PA5-18237) and Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to KPNA2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-KPNA2 Polyclonal Antibody (Product # PA5-18237) on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), A549 (Lane 2), HeLa (Lane 3), NIH/3T3 (Lane 4), PC-12 (Lane 5), LNCaP (Lane 6), Mouse Testis (Lane 7), Mouse Thymus (Lane 8), Mouse colon (Lane 9), Mouse Spleen (Lane 10) and Rat Testis (Lane 11). A 57.86 kDa band corresponding to KPNA2 was observed except in Mouse colon which is reported to be negative. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000 dilution) and detected by Rabbit anti-Goat IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).