Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27964 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GNAL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (89%), Xenopus laevis (92%), Chicken (95%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.65 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Impaired dopamine- and adenosine-mediated signaling and plasticity in a novel rodent model for DYT25 dystonia.
Yu-Taeger L, Ott T, Bonsi P, Tomczak C, Wassouf Z, Martella G, Sciamanna G, Imbriani P, Ponterio G, Tassone A, Schulze-Hentrich JM, Goodchild R, Riess O, Pisani A, Grundmann-Hauser K, Nguyen HP
Neurobiology of disease 2020 Feb;134:104634
Neurobiology of disease 2020 Feb;134:104634
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNAL using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a GNAL polyclonal antibody (Product # PA5-27964) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of GNAL using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a GNAL polyclonal antibody (Product # PA5-27964) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GNAL Polyclonal Antibody (Product # PA5-27964). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL Polyclonal Antibody (Product # PA5-27964) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using GNAL Polyclonal Antibody (Product # PA5-27964). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL Polyclonal Antibody (Product # PA5-27964) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- GNAL Polyclonal Antibody detects GNAL protein at cytosol on NCI-N87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded NCI-N87 xenograft. GNAL Polyclonal Antibody (Product # PA5-27964) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.