V9591

antibody from NSJ Bioreagents

Targeting: IDO2 INDOL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Antibody Data

Product number

V9591

Provider

NSJ Bioreagents

Product name

INDOL1 Antibody / IDO2

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

IDO2/2638

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Aliquot the INDOL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse NIH 3T3 cell lysate with INDOL1 antibody. Expected molecular weight: ~47 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human placental tissue with INDOL1 antibody (clone IDO2/2638) at 2ug/ml in PBS for 30min RT. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using INDOL1 antibody (clone IDO2/2638). These results demonstrate the foremost specificity of the IDO2/2638 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.