20305-1-AP

antibody from Proteintech Group

Targeting: CD47 IAP, MER6, OA3

Provider product page for 20305-1-AP

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Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [55]
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Validations
Western blot [1]

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Product number: 20305-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20305-1-AP, RRID:AB_10732838
Product name: CD47 antibody
Antibody type: Polyclonal
Description: CD47 antibody (Cat. #20305-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

CD47 protein structure - 20305-1-AP shown in red.

Submitted references PRDM1+ Malignant Cells Mediate an Immunosuppressive Landscape and Resistance to Neoadjuvant Chemoradiotherapy and Immunotherapy in Esophageal Squamous Cell Carcinoma.

Shen D, Li R, She Y, Liu X, Huang Y, Ji Y, Chen K, Song Z, Chen R, Li X, Zhao Q, Chen Q, Chen M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Mar;13(17):e15207

Development of Au(x)Cu(y)Pd(z) Nanocomposites as Therapeutic Agents: Enhancing Cancer Treatment through Autophagy Modulation and Immune-Associated Effects.

Yang LX, Chiu YC, Chen YL, Chen TY, Tsai YT, Chin YC, Yeh YL, Wang YJ, Huang CC, Chen RJ, Liao MY
ACS applied materials & interfaces 2026 Mar 18;18(10):14540-14557

MYC promotes the progression of prostate cancer by regulating CD47 to induce an immunosuppressive microenvironment.

Xin S, Su J, Zhao L, Li R, Li G, Qin W, Zhang Z, Wang C, Zhu Y, Feng L, Sun X, Jin L, Zhai T, Mei W, Gao Z
Cellular and molecular life sciences : CMLS 2026 Mar 11;83(1)

UBQLN1 Inhibition reduces MASH progression through downregulating SIKE/p38 MAPK pathway in hepatocyte.

Chen Y, Yang F, Zheng G, Wang Y, Liu L, Yan Y
Journal of nanobiotechnology 2026 Mar 11;24(1)

PET/CT imaging of the peptide-based anti-CD47 probe [(18)F]AlF-NOTA-Pep-20 in preclinical models.

Bao G, Tang M, Dong Y, Li H, Wang S
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2026 Jun 1;221:107528

Ubiquitination and degradation of CD47 enhances macrophage phagocytosis of hemolytic erythrocytes.

Wang Y, Wu X, Li X, Liu Z, An N, Gu S, He Y, He M, Yin D, Chen Y, Hu X
iScience 2026 Jan 16;29(1):114499

Ir(III) Complexes Convert Cold to Hot Tumors via Ferroptosis/Necroptosis-Driven Immunogenic Cell Death and Photosensitized CD47 Downregulation.

Yu LB, Wang P, Shen QH, Guan QX, Li ZY, Han YY, Zhang XY, Hu QY, Tan CP
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Feb;13(8):e14256

Macrophage-Mimicking Biomimetic Microbubbles for Early Ultrasound Molecular Imaging of Acute Rejection in Heart Transplantation.

Wang C, Li G, Chen J, Liu C, Liu Y, Ai X, Yang Y, Leng X, Wang Z, Jiang S
ACS applied materials & interfaces 2026 Feb 4;18(4):6639-6655

A multidimensional pan-cancer analysis of CD47 and its role in promoting malignant phenotype in pancreatic adenocarcinoma.

Su S, Wu B, Li Y, Wang X, Lou F, Mei Z, Guo L
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2026 Feb 16;

Spontaneous Induced Cascade Targeting Biomimetic Nanoparticles to Inhibit Dendritic Cell Maturation for Ameliorating Atherosclerosis and Magnetic Resonance Imaging.

Li D, Chang P, Bian S, Li B, Zhu Y, Wang Y, Hou P, Li J
Biomaterials research 2025;29:0204

Evodiamine protects against cardiac remodeling in heart failure through modulation of calcium signaling and CD47.

Guo L, Niu H, Li J, Shao X, Li N, Sun Y, Ma Y, Wang H
Biochemical and biophysical research communications 2025 Sep 1;777:152168

Expression of Signal Regulatory Protein Alpha in Tumor Cells is the Key Factor in Intrahepatic Cholangiocarcinoma.

Toshida K, Itoh S, Nakayama Y, Yugawa K, Ishikawa T, Tsutsui Y, Tomiyama T, Iseda N, Fujiyoshi S, Motomura T, Toshima T, Iwasaki T, Oda Y, Yoshizumi T
Annals of surgical oncology 2025 Oct;32(10):8037-8045

Inhibition of Skp2 enhances anti-tumor immunity of macrophages by regulating CD47 in gastric cancer.

Hu K, Zhao L, Miao P, Qiu W, Ai L, Chen X, Wang SQ, Chen X, Shi XJ
International immunopharmacology 2025 Oct 10;163:115262

Dual-Targeted Biomimetic Nanoparticles for Enhanced Delivery of Polyphyllin B Synergistically Induce Ferroptosis and Immunogenic Cell Death in Gastric Cancer.

Zu D, Yao J, Li X, Huang Y, Hu C, Deng M, Bao Q, Shi Y, Ye Z, Cheng X
ACS applied materials & interfaces 2025 Nov 12;17(45):61676-61694

Cancer vaccine from intracellularly gelated tumor cells functionalized with CD47 blockage and damage-associated molecular pattern exposure.

Gao C, Luo R, Kwong CHT, Liu J, Tang M, Xie B, Duan T, Wang R
Cell reports. Medicine 2025 May 20;6(5):102092

Inflammation-responsive biomimetic nanoparticles with epigallocatechin-3-gallate for acute lung injury therapy via autophagy enhancement.

Han Y, Liu T, Wei R, Dai C, Huang X, Hu D
iScience 2025 May 16;28(5):112318

USP2 inhibition unleashes CD47-restrained phagocytosis and enhances anti-tumor immunity.

Dai P, Sun Y, Huang Z, Liu YT, Gao M, Liu HM, Shi J, He C, Xiang B, Yao Y, Yu H, Xu G, Kong L, Xiao X, Wang X, Zhang X, Xiong W, Hu J, Lin D, Zhong B, Chen G, Gong Y, Xie C, Zhang J
Nature communications 2025 May 16;16(1):4564

GW501516 facilitated tumor immune escape by inhibiting phagocytosis.

Qian J, Guo Y, Khan B, Shi J, Hou Y
European journal of pharmacology 2025 May 15;995:177418

CD47 Contributes to the Proliferation of Breast Cancer.

Wang J, Wu X, Liu X, Xu Y
Frontiers in bioscience (Landmark edition) 2025 Mar 5;30(3):28210

Endoscopic nasal delivery of engineered endothelial progenitor cell-derived exosomes improves angiogenesis and neurological deficits in rats with intracerebral hemorrhage.

Wan G, Li Z, Gu L, Sun Y, Wang Y, Wang Y, Geng R, Chen Y, Ma W, Bao X, Wang R
Materials today. Bio 2025 Jun;32:101652

Platelet membrane-cloaked biomimetic nanoparticles for targeted acute lung injury therapy.

Lin P, Gao R, Yang W, Fang Z, Wang Z, Yu M, Xu L, Ma Z, Fang J, Yu W
Colloids and surfaces. B, Biointerfaces 2025 Jun;250:114542

Paclitaxel-Ang-2-functionalized bionic mesoporous selenium nanoparticles for targeted therapy of glioma.

Shi H, Wang B, Shi Z, Ma H, Li Y, Liu Y, Zhao Y, Xia N, Wu C, Gao Y
Pharmacological research 2025 Jun;216:107783

PYGO1 drives gastric cancer progression via the ITGB1/CD47 axis and is therapeutically targeted by pentagalloylglucose.

Jia Y, Li Y, Li Y, Li Y, Qu T, Fu Z, Ma Y, Li Z, Wang W, Yu M, Jin X, Gao X, Liu Y
Journal of translational medicine 2025 Jul 29;23(1):852

PPARδ Antagonist Inhibited CD47 Expression and Phagocytosis.

Guo Y, Khan B, Shi J, Hou Y
Journal of cellular biochemistry 2025 Jan;126(1):e30685

Targeted biomimetic fusogenic liposome enhances tumor accumulation, penetration, and therapeutic efficacy of siRNA therapeutics.

Zhao X, Wu K, Zhang Y, Zhang H, Zhang X, Ni Q, Wang Z, Xia Y
Materials today. Bio 2025 Dec;35:102414

Overexpressed CD24 and CD47 Indicate a Worse Prognosis in Cervical Cancer.

Yu M, Ni M, Xu F, Fang C, Li J, Wu X, Ke G
Cancer medicine 2025 Dec;14(23):e71443

Targeting the CD47-HCK-LGALS9 axis disrupts proliferation-immunosuppression coupling in early-stage endometrial cancer.

Ye J, Yan Y, Sun X, Wu Q, Shi Y, Zhang M, Lin X, Ren Y, Mao X, Lin M, Sun P
Molecular cancer 2025 Dec 23;25(1)

Elevated CD47 Expression Impairs Elimination of Photoaged Fibroblasts by Macrophages and Serves as a Potential Biomarker for Photoaging.

Xu X, Lu X, Chen X, Yao A, Lai W
Journal of cosmetic dermatology 2025 Apr;24(4):e70098

CpG-Based Nanovaccines Enhance Ovarian Cancer Immune Response by Gbp2-Mediated Remodeling of Tumor-Associated Macrophages.

Xiong J, Huang J, Xu H, Wu Q, Zhao J, Chen Y, Fan G, Guan H, Xiao R, He Z, Wu S, Ouyang W, Wang S, Zhang L, Xia P, Zhang W, Wu M
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Apr;12(15):e2412881

THBS2-producing matrix CAFs promote colorectal cancer progression and link to poor prognosis via the CD47-MAPK axis.

Liu Z, Ba Y, Shan D, Zhou X, Zuo A, Zhang Y, Xu H, Liu S, Liu B, Zhao Y, Weng S, Wang R, Deng J, Luo P, Cheng Q, Hu X, Yang S, Wang F, Han X
Cell reports 2025 Apr 22;44(4):115555

Bio-Inspired Nanodelivery Platform: Platelet Membrane-Cloaked Genistein Nanosystem for Targeted Lung Cancer Therapy.

Gao R, Lin P, Yang W, Fang Z, Gao C, Cheng B, Fang J, Yu W
International journal of nanomedicine 2024;19:10455-10478

Biomimetic nanomedicine cocktail enables selective cell targeting to enhance ovarian Cancer chemo- and immunotherapy.

Dong Z, Yang W, Zhang Y, Wang B, Wan X, Li M, Chen Y, Zhang N
Journal of controlled release : official journal of the Controlled Release Society 2024 Sep;373:172-188

Dual-targeted halofuginone hydrobromide nanocomplexes for promotion of macrophage repolarization and apoptosis of rheumatoid arthritis fibroblast-like synoviocytes in adjuvant-induced arthritis in rats.

Zhu J, Lin Y, Li G, He Y, Su Z, Tang Y, Zhang Y, Xu Q, Yao Z, Zhou H, Liu B, Cai X
Journal of pharmaceutical analysis 2024 Nov;14(11):100981

CD47, a novel YAP target gene, contributes to hepatic stellate cell activation and liver fibrosis induced by high-fat diet.

Li Y, Dong L, Yin X, Wang X, Zhu X, Zheng P, Tang Y
Heliyon 2024 May 30;10(10):e31621

Efficient Delivery of Lomitapide using Hybrid Membrane-Coated Tetrahedral DNA Nanostructures for Glioblastoma Therapy.

Song M, Tian J, Wang L, Dong S, Fu K, Chen S, Liu C
Advanced materials (Deerfield Beach, Fla.) 2024 Jun;36(24):e2311760

Biomimetic platelet-like nanoparticles enhance targeted hepatocellular carcinoma therapy.

Wu Y, Fan Q, Zhou J, Hu H, Liao Z, Tang X, Xu M, Yang S, Lai J, Wan S, Wu J
Colloids and surfaces. B, Biointerfaces 2024 Aug;240:113973

Canadine Platinum(IV) Complexes Targeting Epithelial-Mesenchymal Transition as Antiproliferative and Antimetastatic Agents.

Chen Y, Zhang M, He Y, Li S, Feng S, Liu Z, Zhang N, Liu M, Wang Q
Journal of medicinal chemistry 2024 Aug 8;67(15):12868-12886

Targeting and repolarizing M2-like tumor-associated macrophage-mediated MR imaging and tumor immunotherapy by biomimetic nanoparticles.

Chong L, Jiang YW, Wang D, Chang P, Xu K, Li J
Journal of nanobiotechnology 2023 Oct 31;21(1):401

Engineering high-affinity dual targeting cellular nanovesicles for optimised cancer immunotherapy.

Zhang L, Zhao X, Niu Y, Ma X, Yuan W, Ma J
Journal of extracellular vesicles 2023 Nov;12(11):e12379

Immunostimulation with chemotherapy of a ruthenium-arene complex via blockading CD47 signal in chronic myelogenous leukemia cells.

Hu G, Lv M, Guo B, Huang Y, Su Z, Qian Y, Xue X, Liu HK
Journal of inorganic biochemistry 2023 Jun;243:112195

RAGA prevents tumor immune evasion of LUAD by promoting CD47 lysosome degradation.

Zhang L, Yu J, Zheng M, Zhen H, Xie Q, Zhang C, Zhou Z, Jin G
Communications biology 2023 Feb 23;6(1):211

α5-nAChR/STAT3/CD47 axis contributed to nicotine-related lung adenocarcinoma progression and immune escape.

Kang G, Jiao Y, Pan P, Fan H, Li Q, Li X, Li J, Wang Y, Jia Y, Wang J, Sun H, Ma X
Carcinogenesis 2023 Dec 15;44(10-11):773-784

PPARγ agonist inhibits c-Myc-mediated colorectal cancer tumor immune escape.

Xu L, Che S, Chen H, Liu Q, Shi J, Jin J, Hou Y
Journal of cellular biochemistry 2023 Aug;124(8):1145-1154

MiR-133a/CD47 axis is a novel prognostic biomarker to promote triple negative breast cancer progression.

Yan H, Huang W, Chen C, Zhang X, Zhu K, Yuan J
Pathology, research and practice 2023 Apr;244:154400

CD47 interactions with exportin-1 limit the targeting of m(7)G-modified RNAs to extracellular vesicles.

Kaur S, Saldana AC, Elkahloun AG, Petersen JD, Arakelyan A, Singh SP, Jenkins LM, Kuo B, Reginauld B, Jordan DG, Tran AD, Wu W, Zimmerberg J, Margolis L, Roberts DD
Journal of cell communication and signaling 2022 Sep;16(3):397-419

Engineered biomimetic nanoreactor for synergistic photodynamic-chemotherapy against hypoxic tumor.

Guo H, Wang L, Wu W, Guo M, Yang L, Zhang Z, Cao L, Pu F, Huang X, Shao Z
Journal of controlled release : official journal of the Controlled Release Society 2022 Nov;351:151-163

Biomimetic cell membrane-coated glucose/oxygen-exhausting nanoreactor for remodeling tumor microenvironment in targeted hypoxic tumor therapy.

Guo H, Zhang W, Wang L, Shao Z, Huang X
Biomaterials 2022 Nov;290:121821

Bioinspired Platelet-like Nanovector for Enhancing Cancer Therapy via P-Selectin Targeting.

Wan S, Wu Y, Fan Q, Yang G, Hu H, Tima S, Chiampanichayakul S, Anuchapreeda S, Wu J
Pharmaceutics 2022 Nov 26;14(12)

Extracellular matrix modulating enzyme functionalized biomimetic Au nanoplatform-mediated enhanced tumor penetration and synergistic antitumor therapy for pancreatic cancer.

Yang XY, Zhang JG, Zhou QM, Yu JN, Lu YF, Wang XJ, Zhou JP, Ding XF, Du YZ, Yu RS
Journal of nanobiotechnology 2022 Dec 10;20(1):524

Combinatorial Analysis of AT-Rich Interaction Domain 1A and CD47 in Gastric Cancer Patients Reveals Markers of Prognosis.

Zhao Q, Cai Q, Yu S, Ji J, Zhu Z, Yan C, Zhang J
Frontiers in cell and developmental biology 2021;9:745120

Bioengineering CXCR4-overexpressing cell membrane functionalized ROS-responsive nanotherapeutics for targeting cerebral ischemia-reperfusion injury.

Luo L, Zang G, Liu B, Qin X, Zhang Y, Chen Y, Zhang H, Wu W, Wang G
Theranostics 2021;11(16):8043-8056

Combination of CD47 and CD68 expression predicts survival in eastern-Asian patients with non-small cell lung cancer.

Fu F, Zhang Y, Gao Z, Zhao Y, Wen Z, Han H, Li Y, Hu H, Chen H
Journal of cancer research and clinical oncology 2021 Mar;147(3):739-747

4T1 cell membrane fragment reunited PAMAM polymer units disguised as tumor cell clusters for tumor homotypic targeting and anti-metastasis treatment.

Pei X, Pan X, Xu X, Xu X, Huang H, Wu Z, Qi X
Biomaterials science 2021 Feb 23;9(4):1325-1333

Identification of Schlafen-11 as a Target of CD47 Signaling That Regulates Sensitivity to Ionizing Radiation and Topoisomerase Inhibitors.

Kaur S, Schwartz AL, Jordan DG, Soto-Pantoja DR, Kuo B, Elkahloun AG, Mathews Griner L, Thomas CJ, Ferrer M, Thomas A, Tang SW, Rajapakse VN, Pommier Y, Roberts DD
Frontiers in oncology 2019;9:994

Aptamer-Based Erythrocyte-Derived Mimic Vesicles Loaded with siRNA and Doxorubicin for the Targeted Treatment of Multidrug-Resistant Tumors.

Wang T, Luo Y, Lv H, Wang J, Zhang Y, Pei R
ACS applied materials & interfaces 2019 Dec 11;11(49):45455-45466

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The CD47 antibody from Proteintech is a rabbit polyclonal antibody to a fusion protein of human CD47. This antibody recognizes human antigen. The CD47 antibody has been validated for the following applications: ELISA, WB analysis.
Appendix: 20305-1-AP.pdf

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