Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF4670 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal CD47 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD47 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD47 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Combined prognostic value of the cancer stem cell markers CD47 and CD133 in esophageal squamous cell carcinoma.
Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment.
Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
Wang JH, Huang ST, Zhang L, Liu ZG, Liang RX, Jiang SW, Jiang YN, Yu XJ, Jiang YC, Li XZ, Zhang PF, Wen ZS, Zheng M
Cancer medicine 2019 Mar;8(3):1315-1325
Cancer medicine 2019 Mar;8(3):1315-1325
Relationship between tumor-associated macrophage subsets and CD47 expression in squamous cell carcinoma of the head and neck in the tumor microenvironment.
Sakakura K, Takahashi H, Kaira K, Toyoda M, Murata T, Ohnishi H, Oyama T, Chikamatsu K
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):994-1003
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):994-1003
Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay.
Baccelli I, Schneeweiss A, Riethdorf S, Stenzinger A, Schillert A, Vogel V, Klein C, Saini M, Bäuerle T, Wallwiener M, Holland-Letz T, Höfner T, Sprick M, Scharpff M, Marmé F, Sinn HP, Pantel K, Weichert W, Trumpp A
Nature biotechnology 2013 Jun;31(6):539-44
Nature biotechnology 2013 Jun;31(6):539-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human CD47 by Western Blot. Detection of Human CD47 by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- CD47 in Human Placenta. CD47 in Human Placenta.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of CD47 in Human Lymphocytes by Flow Cytometry. Detection of CD47 in Human Lymphocytes by Flow Cytometry.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by SIRPa/CD172a and Neutral-ization by Human CD47 Antibody. Cell Adhesion Mediated by SIRPa/CD172a and Neutral-ization by Human CD47 Antibody.