Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 323102 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#323102, RRID:AB_756132
- Product name
- Purified anti-human CD47
- Antibody type
- Monoclonal
- Antigen
- CCRF-CEM T-cell line
- Description
- CD47 also known as Rh-associated protein, gp42, integrin-associated protein (IAP), and neurophilin, is a 42-52 kD member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail, the shorter form is expressed in bone-marrow-derived cells, endothelial cells, and fibroblasts while the longer form is expressed by neural tissues. CD47 expression is widely distributed in hematopoietic cells including thymocytes, T cells, B cells, monocytes, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues. CD47 functions as an adhesion molecule and thrombospondin receptor and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47; in the absence of CD47 mice show defects in host defense and β3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is also part of the Rh complex on erythrocytes. The CC2C6 antibody recognizes human CD47 and has been shown to be useful for flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Hypoxic exercise training causes erythrocyte senescence and rheological dysfunction by depressed Gardos channel activity.
Mao TY, Fu LL, Wang JS
Journal of applied physiology (Bethesda, Md. : 1985) 2011 Aug;111(2):382-91
Journal of applied physiology (Bethesda, Md. : 1985) 2011 Aug;111(2):382-91
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes stained with purified CC2C6, followed by anti-mouse IgGs FITC
- Conjugate
- Unconjugated