Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB4670 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD47 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD47. Stains human CD47 transfectants but not irrelevant transfectants.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q08722- Isotype
- IgG
- Antibody clone number
- 472603
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma.
Ye X, Wang X, Lu R, Zhang J, Chen X, Zhou G
Oncology letters 2018 Jun;15(6):9075-9080
Oncology letters 2018 Jun;15(6):9075-9080
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD47 in Human Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SIRP alpha/CD172a Binding to CD47-transfected HEK293 Human Cell Line is Blocked by Human CD47 Antibody. In a functional flow cytometry test, biotinylated recombinant Human SIRP alpha/CD172a (100 ng/mL, Catalog # 4546-SA) binds to Human CD47-transfected HEK293 human embryonic kidney cell line (black dotted line). Binding is completely blocked (orange histogram) by 2.5 μg/mL of Mouse Anti-Human CD47 Monoclonal Antibody (Catalog # MAB4670). Mouse IgG1 isotype (Catalog # MAB002) at 2.5 μg/mL was used as a control (blue line). Cells were stained with Streptavidin-APC (Catalog # F0050).
