Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF5059 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal AP-2 gamma Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human AP-2 gamma in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) AP-2 alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Trophoblast organoids as a model for maternal-fetal interactions during human placentation.
Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.
Turco MY, Gardner L, Kay RG, Hamilton RS, Prater M, Hollinshead MS, McWhinnie A, Esposito L, Fernando R, Skelton H, Reimann F, Gribble FM, Sharkey A, Marsh SGE, O'Rahilly S, Hemberger M, Burton GJ, Moffett A
Nature 2018 Dec;564(7735):263-267
Nature 2018 Dec;564(7735):263-267
Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.
Latos PA, Sienerth AR, Murray A, Senner CE, Muto M, Ikawa M, Oxley D, Burge S, Cox BJ, Hemberger M
Genes & development 2015 Dec 1;29(23):2435-48
Genes & development 2015 Dec 1;29(23):2435-48
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human AP-2 gamma by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line and human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2 gamma at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- AP-2 gamma in Human Breast Cancer Tissue. AP-2 gamma was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 10 µg/mL Goat Anti-Human AP-2 gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5059) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.