14572-1-AP

antibody from Invitrogen Antibodies

Targeting: TFAP2C AP2-GAMMA, ERF1, hAP-2g, TFAP2G

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14572-1-AP

Provider

Invitrogen Antibodies

Product name

TFAP2C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MLWKITDNV KYEEDCEDRH DGSSNGNPRV PHLSSAGQHL YSPAPPLSHT GVAEYQPPPY FPPPYQQLAY SQSADPYSHL GEAYAAAINP LHQPAPTGSQ QQAWPGRQSQ EGAGLPSHHG RPAGLLPHLS GLEAGAVSAR RDAYRRSDLL LPHAHALDAA GLAENLGLHD MPHQMDEVQN VDDQHLLLHD QTVIRKGPIS MTKNPLNLPC QKELVGAVMN PTEVFCSVPG RLSLLSSTSK YKVTVAEVQR RLSPPECLNA SLLGGVLRRA KSKNGGRSLR EKLDKIGLNL PAGRRKAAHV TLLTSLVEGE AVHLARDFAY VCEAEFPSKP VAEYLTRPHL GGRNEMAARK NMLLAAQQLC KEFTELLSQD RTPHGTSRLA PVLETNIQNC LSHFSLITHG FGSQAICAAV SALQNYIKEA LIVIDKSYMN PGDQSPADSN KTLEKMEKHR K (1-450 aa encoded by BC035664)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.23 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human placenta tissue were subjected to SDS PAGE followed by western blot with 14572-1-AP (TFAP2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14572-1-AP ( TFAP2C Antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 14572-1-AP (TFAP2C antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14572-1-AP (TFAP2C Antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 MCF-7 cells were stained with 0.2ug TFAP2C antibody (14572-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-TFAP2C (IP:14572-1-AP, 4ug; Detection:14572-1-AP 1:1000) with HeLa cells lysate 2800ug.