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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- NBP2-59682-100ug - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal HSF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Porcine, Rabbit, Sheep, Simian
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HSF2 Antibody (3E2) [NBP2-59682] - Analysis of Human K562 cell lysates showing detection of HSF2 protein using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:1000. Cells transiently transfected with control, HSF1 or HSF2 shRNA constructs. Courtesy of: Lea Sistonen, Abo Akademi University, Finland.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HSF2 Antibody (3E2) [NBP2-59682] - Analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: HSF2 Antibody (3E2) [NBP2-59682] - Analysis using Rat Anti-HSF2 Monoclonal Antibody, Clone 3E2 . Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF2 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF2 Antibody. (C) Composite.
