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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-96200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: 293T, HepG2, SW620, BT-474; Cellular Location: Cytoplasm, Nucleus Immunogen sequence: LDEQRFAKEI LPKYFKHNNM ASFVRQLNMY GFRKVVHIDS GIVKQERDGP VEFQHPYFKQ GQDDLLENIK RKVSSSKPEE NKIRQEDLTK IISSAQKVQI KQETIESRLS ELKSENESLW KEVSELRAKH AQQQQVIRKI VQFIVTLVQN NQLVSLKRKR PLLLNTNGAQ KKNLFQH
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.46 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HSF2 in extracts of various cell lines using HSF2 Polyclonal Antibody (Product # PA5-96200) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HSF2 was performed in A549 cells using HSF2 Polyclonal Antibody (Product # PA5-96200).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HSF2 was performed in A549 cells using HSF2 Polyclonal Antibody (Product # PA5-96200).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of HSF2 in paraffin-embedded rat liver using HSF2 Polyclonal Antibody (Product # PA5-96200) at a dilution of 1:200.
