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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
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- Product number
- PA5-30064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HSF2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, mouse liver. Predicted reactivity: Mouse (98%), Rat (98%), Xenopus laevis (81%), Chicken (87%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HSF2 using 30 µg A549 whole cell lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a HSF2 polyclonal antibody (Product # PA5-30064) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSF2 Polyclonal Antibody (Product # PA5-30064). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. HSF2 Polyclonal Antibody (Product # PA5-30064) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSF2 Polyclonal Antibody (Product # PA5-30064). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSF2 Polyclonal Antibody (Product # PA5-30064) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HSF2 Polyclonal Antibody (Product # PA5-30064). Untreated (–) and treated (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSF2 Polyclonal Antibody (Product # PA5-30064) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
