MIA1610

antibody from Invitrogen Antibodies

Targeting: APOB

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

MIA1610

Provider

Invitrogen Antibodies

Product name

Anti-Apolipoprotein B Monoclonal Antibody (F2C13)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MIA1610 targets Apolipoprotein B in ELISA, IP, and RIA applications and shows reactivity with Human samples. The MIA1610 immunogen is purified human serum LDL. MIA1610 detects Apolipoprotein B which has a predicted molecular weight of approximately 513 kDa. MIA1610 was formerly sold as a Seradyn product.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

F2C13

Vial size

1 mg

Concentration

5 mg/mL

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of Apolipoprotein B was done on Hep G2 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with Apolipoprotein B Mouse Monoclonal Antibody (MIA1610, red histogram) or with mouse isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Rabbit Anti-Mouse Secondary Antibody (A11059) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..