Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF3556 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Apolipoprotein B/ApoB Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Filter-Dense Multicolor Microscopy.
Scavenger receptor-BI is a receptor for lipoprotein(a).
Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin.
Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P
PloS one 2015;10(3):e0119499
PloS one 2015;10(3):e0119499
Scavenger receptor-BI is a receptor for lipoprotein(a).
Yang XP, Amar MJ, Vaisman B, Bocharov AV, Vishnyakova TG, Freeman LA, Kurlander RJ, Patterson AP, Becker LC, Remaley AT
Journal of lipid research 2013 Sep;54(9):2450-7
Journal of lipid research 2013 Sep;54(9):2450-7
Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin.
Nahmias Y, Goldwasser J, Casali M, van Poll D, Wakita T, Chung RT, Yarmush ML
Hepatology (Baltimore, Md.) 2008 May;47(5):1437-45
Hepatology (Baltimore, Md.) 2008 May;47(5):1437-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Apolipoprotein B/ApoB by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Apolipoprotein B/ApoB in Human Liver. Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.