AF3556

antibody from Novus Biologicals

Targeting: APOB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3556

Provider

Novus Biologicals

Product name

Goat Polyclonal Apolipoprotein B/ApoB Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Apolipoprotein B/ApoB in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Filter-Dense Multicolor Microscopy.

Kijani S, Yrlid U, Heyden M, Levin M, Borén J, Fogelstrand P
PloS one 2015;10(3):e0119499

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Scavenger receptor-BI is a receptor for lipoprotein(a).

Yang XP, Amar MJ, Vaisman B, Bocharov AV, Vishnyakova TG, Freeman LA, Kurlander RJ, Patterson AP, Becker LC, Remaley AT
Journal of lipid research 2013 Sep;54(9):2450-7

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Apolipoprotein B-dependent hepatitis C virus secretion is inhibited by the grapefruit flavonoid naringenin.

Nahmias Y, Goldwasser J, Casali M, van Poll D, Wakita T, Chung RT, Yarmush ML
Hepatology (Baltimore, Md.) 2008 May;47(5):1437-45

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Apolipoprotein B/ApoB by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B/ApoB at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Apolipoprotein B/ApoB in Human Liver. Apolipoprotein B/ApoB was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Apolipoprotein B/ApoB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3556) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm and plasma membrane of hepatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.