LS-C355399

antibody from LSBio

Targeting: APOB

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

LS-C355399

Provider

LSBio

Product name

APOB / Apolipoprotein B Antibody (clone F2C3) LS-C355399

Antibody type

Monoclonal

Description

Ion exchange chromatography

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

F2C3

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry analysis of Apolipoprotein B showing staining in the cytoplasm of paraffin-embedded human liver tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Apolipoprotein B Mouse Monoclonal Antibody diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.