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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF3260 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Apolipoprotein B100/ApoB100 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human ApoB100 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAP72970- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Intimal hyperplasia induced by vascular intervention causes lipoprotein retention and accelerated atherosclerosis.
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
Kijani S, Vázquez AM, Levin M, Borén J, Fogelstrand P
Physiological reports 2017 Jul;5(14)
Physiological reports 2017 Jul;5(14)
MicroRNA-27a regulates lipid metabolism and inhibits hepatitis C virus replication in human hepatoma cells.
Shirasaki T, Honda M, Shimakami T, Horii R, Yamashita T, Sakai Y, Sakai A, Okada H, Watanabe R, Murakami S, Yi M, Lemon SM, Kaneko S
Journal of virology 2013 May;87(9):5270-86
Journal of virology 2013 May;87(9):5270-86
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Apolipoprotein B100/ApoB100 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Apolipoprotein B100/ApoB100 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein B100/ApoB100 at approximately 500 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
