MA5-16079

antibody from Invitrogen Antibodies

Targeting: ABCC1 GS-X, MRP, MRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-16079

Provider

Invitrogen Antibodies

Product name

MRP1 Monoclonal Antibody (IU5C1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

The target sequence has 96% sequence homology with rat, primate, and feline, 88% sequence homology with chicken, 87% sequence homology with bovine, 72% sequence homology with drosophila melanogaster.

Antibody clone number

IU5C1

Concentration

1.0 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRP1 using a monoclonal antibody (Product # MA5-16079).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MRP1 in 10 µg 293 transfected and empty vector lysate. Samples were incubated in MRP1 monoclonal antibody (Product # MA5-16079). Lane 1: empty vector lysate; Lane 2: 293 transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MRP1 Monoclonal Antibody (IU5C1) (Product # MA5-16079) and a 150-220 kDa band corresponding to ABCC1 was observed across tested tissues. Tissue extracts (50 µg lysate) of Mouse Skeletal Muscle (Lane 1), Rat Skeletal Muscle (Lane 2), Mouse Brain (Lane 3), Mouse Testis (Lane 4), Mouse Liver (Lane 5) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:300) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of MRP1 in HEK293 cells with MRP1 expression. Samples were incubated in MRP1 monoclonal antibody (Product # MA5-16079).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of MRP1 in formalin-fixed paraffin-embedded tissue section of mouse testis. Samples were incubated in MRP1 monoclonal antibody (Product # MA5-16079) using a dilution of 1:200 followed by HRP labeled anti-mouse secondary antibody and DAB reagent. Nuclei of cells were counter-stained with hematoxylin.