53-0194-80

antibody from Invitrogen Antibodies

Targeting: CD19

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

53-0194-80

Provider

Invitrogen Antibodies

Product name

CD19 Monoclonal Antibody (6OMP31), Alexa Fluor™ 488, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: The 6OMP31 monoclonal antibody reacts with human, mouse, and rat CD19. CD19 is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. CD19 was first identified as the B4 antigen of human B lymphocytes using the anti-B4 monoclonal. It is expressed by B cells during all stages of development, except on terminally differentiated plasma cells, and on follicular dendritic cells. CD19 acts primarily as a B cell co-receptor and together with CD21, CD81, Leu13, and MHC class II forms a multi-molecular complex that associates with the BCR. CD19 plays a role in the development, activation, differentiation, and proliferation of B cells. CD19 deficiency leads to an overall impaired humoral immune response. Mutations in CD19 have been associated with severe immunodeficiency syndromes. Although it is unclear whether CD19 contributes directly to B cell carcinogenesis, it is expressed on most B cell tumors, such as acute lymphoblastic leukemias and B cell lymphomas. The use of CD19 monoclonal antibodies for therapies against lymphoma, leukemia and autoimmune disorders are currently being explored. Applications Reported: This 6OMP31 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 6OMP31 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low or high pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.

Reactivity

Human, Mouse, Rat

Host

Rat

Conjugate

Green dye

Isotype

IgG

Antibody clone number

6OMP31

Vial size

25 µg

Concentration

0.5 mg/mL

Storage

4°C, store in dark, DO NOT FREEZE!

References

Submitted references

Knockdown of CD146 promotes endothelial-to-mesenchymal transition via Wnt/β-catenin pathway.

Zhang ZY, Zhai C, Yang XY, Li HB, Wu LL, Li L
PloS one 2022;17(8):e0273542

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Fecal microbiota transplantation ameliorates atherosclerosis in mice with C1q/TNF-related protein 9 genetic deficiency.

Kim ES, Yoon BH, Lee SM, Choi M, Kim EH, Lee BW, Kim SY, Pack CG, Sung YH, Baek IJ, Jung CH, Kim TB, Jeong JY, Ha CH
Experimental & molecular medicine 2022 Feb;54(2):103-114

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Distribution of Immune Cells Including Macrophages in the Human Cochlea.

Liu W, Danckwardt-Lillieström N, Schrott-Fischer A, Glueckert R, Rask-Andersen H
Frontiers in neurology 2021;12:781702

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Single-cell evaluation reveals shifts in the tumor-immune niches that shape and maintain aggressive lesions in the breast.

Sinha VC, Rinkenbaugh AL, Xu M, Zhou X, Zhang X, Jeter-Jones S, Shao J, Qi Y, Zebala JA, Maeda DY, McAllister F, Piwnica-Worms H
Nature communications 2021 Aug 18;12(1):5024

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Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice.

Tashita C, Hoshi M, Hirata A, Nakamoto K, Ando T, Hattori T, Yamamoto Y, Tezuka H, Tomita H, Hara A, Saito K
World journal of gastroenterology 2020 Mar 7;26(9):918-932

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Complement Signals Determine Opposite Effects of B Cells in Chemotherapy-Induced Immunity.

Lu Y, Zhao Q, Liao JY, Song E, Xia Q, Pan J, Li Y, Li J, Zhou B, Ye Y, Di C, Yu S, Zeng Y, Su S
Cell 2020 Mar 19;180(6):1081-1097.e24

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A novel platform to produce human monoclonal antibodies: The next generation of therapeutic human monoclonal antibodies discovery.

Duvall M, Bradley N, Fiorini RN
mAbs 2011 Mar-Apr;3(2):203-8

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Multiplexed fluorescent western blot was performed using CD19 Monoclonal Antibody (6OMP31), Alexa Fluor 488, eBioscience™ (Product # 53-0194-82) and a ~ 80 kDa band corresponding to CD19 was observed in Raji, Ramos and Daudi which are reported to be positive and not in Jurkat, MOLT-4 and SW480. Membrane enriched extracts (30 µg lysate) of Raji (Lane 1), Ramos (Lane 2), Daudi (Lane 3), Jurkat (Lane 4), MOLT-4 (Lane 5) and SW480 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX), 12 well. Resolved proteins were transferred onto a Low-Fluorescence PVDF Transfer Membrane (Product # 22860) using iBlot® 2 Dry Blotting System (Product # IB21001) and blocked with Blocker™ FL Fluorescent Blocking Buffer (10X) (Product # 37565). The blot was probed with the primary antibody (0.5 µg/mL) and HSP70 Polyclonal Antibody (Product # PA5-28003, 1:4,000). Secondary antibody Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 800 (Product # A32808, 1:10,000) was used for detection of HSP70. Fluorescent detection was performed using iBright™ FL1500 (Product # A44115).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of formalin-fixed paraffin embedded human tonsil using 10 µg/mL Rat IgG2a K Isotype Control Alexa Fluor® 488 (left) or 10 µg/mL Anti-CD19 Alexa Fluor® 488 (right). Nuclei are counterstained with DAPI.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 The aggressive niche harbors an expanded and spatially restricted myeloid population. a - c UMAP was used for dimension reduction of single-cell data. Immune fractions from indolent and aggressive lesions were computationally merged ( a ) for cluster analysis and identification ( b ). Proportion of clusters per group shown in c . NK natural killer, pDC plasmacytoid dendritic cell, cDC conventional dendritic cell. d Heatmap of top differentially expressed (DE) genes for each cluster, ordered in decreasing cluster size. Clusters indicated by top horizontal bar, with inferred immune identity noted. Cells derived from indolent or aggressive tumor sample indicated by second horizontal bar. DE genes are listed on the left. e Immunohistochemical staining for major lymphoid (T cells--CD3; B cells--CD19) and myeloid (macrophages--F4/80; neutrophils--MPO) populations associated with indolent and aggressive lesions. Arrows point to selected positively stained cells. Representative images shown from n = 4, 3, 3, 4 animals for CD3, CD19, F4/80, and MPO, respectively. f Immunofluorescent staining for neutrophils using an alternative marker S100A8. Left panel shows low power magnification view of adjacent indolent and aggressive lesions, with S100A8+ infiltration tightly localized with the aggressive niche. Boxes correspond by color to high magnification images shown middle (blue, indolent) and right (yellow, aggressive) panels. Representative images shown from n = 7 animals. g Immun

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 13 (A) CD19-positive B lymphocyte in the modiolus. The cell also expresses MHCII. (B) CD4 and CD8-positive T cells in the peripheral region of the RC. (C) A CD8-positive lymphocyte and an MHCII-expressing cell in Rosenthal's canal. (D) A CD8 cell is seen to physically interact with an IBA1-positive macrophage.

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

The accumulation of immune cells results in atherosclerosis progression. a , b Immunofluorescence staining for the lymphocyte marker CD45 (red) and DAPI-stained nuclei (blue) are shown. c , d T cells were stained for CD3. e , f B cells were stained for CD19. g , h Macrophages were stained for CD68. * p < 0.05 for the WT-FMT group and the KO group compared to the WT group, # p < 0.05 for the KO-FMT group compared to the KO group. Two-way comparisons were performed with t tests. Scale bars in right lower corner represent 50 mum.

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

S2 Fig CD146 was not expressed in SMCs and certain immune cells in adult mouse hearts. Representative double-immunofluorescence images of CD146 with alpha-SMA (a marker for SMCs), MPO (a marker for neutrophils), CD68 (a marker for monocytes/macrophages), CD19 (a marker for B lymphocytes), and CD4 (a marker for T-helper cells) in left ventricles from adult mice (scale bars indicate 50mum). Green represents CD146; red represents CD4, CD19, CD68, MPO and alpha-SMA, respectively; blue represents nuclei. (TIF) Click here for additional data file.

Conjugate

Green dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1 Increased number of F4/80 + KMO + cells in the colon exacerbates TNBS-induced colitis. Mice were treated with either TNBS or vehicle, and colons were collected three days after treatment. A: Histological scores determined based on a histological grading as described in Materials and Methods. B: Representative images of H&E-stained colons of the said mice. C, D: The KMO + cells in the colons of the said mice were counted using immunohistochemical staining for KMO. E: KMO mRNA expression in the colon was determined by quantitative PCR. F: The colons of TNBS-treated mice were stained for F4/80, CD19, CD11c, KMO, and DAPI (nuclei) using immunofluorescence staining. The negative controls for F4/80, CD19, CD11c, and KMO were not stained ( Supplementary Figure 2 and 3 ). a P < 0.05 vs Vehicle group, b P < 0.01 vs Vehicle group, c P < 0.05 vs TNBS-treated KMO +/+ mice group. KMO: Kynurenine 3-monooxygenase; TNBS: Trinitrobenzene sulfonic acid.

Conjugate

Green dye