20778-1-AP

antibody from Proteintech Group

Targeting: WDR24 C16orf21, DKFZp434F054, JFP7

Provider product page for 20778-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [23]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 20778-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20778-1-AP, RRID:AB_10696183
Product name: WDR24 antibody
Antibody type: Polyclonal
Description: KD/KO validated WDR24 antibody (Cat. #20778-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

WDR24 protein structure - 20778-1-AP shown in red.

Submitted references The GATOR2 complex maintains lysosomal-autophagic function by inhibiting the protein degradation of MiT/TFEs.

Yang S, Ting CY, Lilly MA
Molecular cell 2024 Feb 15;84(4):727-743.e8

SPOP negatively regulates mTORC1 activity by ubiquitinating Sec13.

Yang Y, Han YC, Cao Q, Wang X, Wei XD, Shang MD, Zhang XG, Li X, Hu B, Tian CY, Yang ZL, Liu KH, Wang JQ
Cellular signalling 2024 Apr;116:111060

Genome-wide CRISPR screens identify ILF3 as a mediator of mTORC1-dependent amino acid sensing.

Yan G, Yang J, Li W, Guo A, Guan J, Liu Y
Nature cell biology 2023 May;25(5):754-764

Role of FLCN Phosphorylation in Insulin-Mediated mTORC1 Activation and Tumorigenesis.

Wang G, Chen L, Lei X, Qin S, Geng H, Zheng Y, Xia C, Yao J, Meng T, Deng L
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2023 Jun;10(17):e2206826

Ring domains are essential for GATOR2-dependent mTORC1 activation.

Jiang C, Dai X, He S, Zhou H, Fang L, Guo J, Liu S, Zhang T, Pan W, Yu H, Fu T, Li D, Inuzuka H, Wang P, Xiao J, Wei W
Molecular cell 2023 Jan 5;83(1):74-89.e9

PRMT1 orchestrates with SAMTOR to govern mTORC1 methionine sensing via Arg-methylation of NPRL2.

Jiang C, Liu J, He S, Xu W, Huang R, Pan W, Li X, Dai X, Guo J, Zhang T, Inuzuka H, Wang P, Asara JM, Xiao J, Wei W
Cell metabolism 2023 Dec 5;35(12):2183-2199.e7

VWCE modulates amino acid-dependent mTOR signaling and coordinates with KICSTOR to recruit GATOR1 to the lysosomes.

Zhao T, Guan Y, Xu C, Wang D, Guan J, Liu Y
Nature communications 2023 Dec 20;14(1):8464

SLC38A2 and glutamine signalling in cDC1s dictate anti-tumour immunity.

Guo C, You Z, Shi H, Sun Y, Du X, Palacios G, Guy C, Yuan S, Chapman NM, Lim SA, Sun X, Saravia J, Rankin S, Dhungana Y, Chi H
Nature 2023 Aug;620(7972):200-208

Effects of Leucine Ingestion and Contraction on the Sestrin/GATOR2 Pathway and mTORC1 Activation in Rat Fast-Twitch muscle.

Kanzaki K, Wada M
The Journal of nutrition 2023 Aug;153(8):2228-2236

TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential.

He J, Lin M, Zhang X, Zhang R, Tian T, Zhou Y, Dong W, Yang Y, Sun X, Dai Y, Xu Y, Zhang Z, Xu M, Lei QY, Xu Y, Lv L
Cell discovery 2023 Aug 8;9(1):84

CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth.

Yin S, Liu L, Ball LE, Wang Y, Bedford MT, Duncan SA, Wang H, Gan W
Cell reports 2023 Apr 25;42(4):112316

Selection-free endogenous tagging of cell lines by bicistronic co-expression of the surface antigen NGFR.

Seibert M, Kurrle N, Stolp V, Nürnberger H, Tzschentke S, Börner L, Wempe F, Serve H, Schnütgen F
MethodsX 2022;9:101929

Structure of the nutrient-sensing hub GATOR2.

Valenstein ML, Rogala KB, Lalgudi PV, Brignole EJ, Gu X, Saxton RA, Chantranupong L, Kolibius J, Quast JP, Sabatini DM
Nature 2022 Jul;607(7919):610-616

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Cangelosi AL, Puszynska AM, Roberts JM, Armani A, Nguyen TP, Spinelli JB, Kunchok T, Wang B, Chan SH, Lewis CA, Comb WC, Bell GW, Helman A, Sabatini DM
Science (New York, N.Y.) 2022 Jul;377(6601):47-56

E3 ligase RNF167 and deubiquitinase STAMBPL1 modulate mTOR and cancer progression.

Wang D, Xu C, Yang W, Chen J, Ou Y, Guan Y, Guan J, Liu Y
Molecular cell 2022 Feb 17;82(4):770-784.e9

GATOR2-dependent mTORC1 activity is a therapeutic vulnerability in FOXO1 fusion-positive rhabdomyosarcoma.

Morales J, Allegakoen DV, Garcia JA, Kwong K, Sahu PK, Fajardo DA, Pan Y, Horlbeck MA, Weissman JS, Gustafson WC, Bivona TG, Sabnis AJ
JCI insight 2022 Dec 8;7(23)

Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation.

Chen X, Yu C, Liu X, Liu B, Wu X, Wu J, Yan D, Han L, Tang Z, Yuan X, Wang J, Wang Y, Liu S, Shan L, Shang Y
Nature communications 2022 Dec 8;13(1):7578

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Long L, Wei J, Lim SA, Raynor JL, Shi H, Connelly JP, Wang H, Guy C, Xie B, Chapman NM, Fu G, Wang Y, Huang H, Su W, Saravia J, Risch I, Wang YD, Li Y, Niu M, Dhungana Y, Kc A, Zhou P, Vogel P, Yu J, Pruett-Miller SM, Peng J, Chi H
Nature 2021 Dec;600(7888):308-313

SAR1B senses leucine levels to regulate mTORC1 signalling.

Chen J, Ou Y, Luo R, Wang J, Wang D, Guan J, Li Y, Xia P, Chen PR, Liu Y
Nature 2021 Aug;596(7871):281-284

Evidence for a role for Sestrin1 in mediating leucine-induced activation of mTORC1 in skeletal muscle.

Xu D, Shimkus KL, Lacko HA, Kutzler L, Jefferson LS, Kimball SR
American journal of physiology. Endocrinology and metabolism 2019 May 1;316(5):E817-E828

CRISPR/Cas9-mediated generic protein tagging in mammalian cells.

Thöne FMB, Kurrle NS, von Melchner H, Schnütgen F
Methods (San Diego, Calif.) 2019 Jul 15;164-165:59-66

Seh1 targets GATOR2 and Nup153 to mitotic chromosomes.

Platani M, Samejima I, Samejima K, Kanemaki MT, Earnshaw WC
Journal of cell science 2018 May 1;131(9)

SZT2 dictates GATOR control of mTORC1 signalling.

Peng M, Yin N, Li MO
Nature 2017 Mar 16;543(7645):433-437

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 20778-1-AP(WDR24 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary using 20778-1-AP(WDR24 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human ovary using 20778-1-AP(WDR24 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue