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LearnNB200-315
antibody from Novus Biologicals
Targeting: HNRNPM
CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB200-315 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-315, RRID:AB_2177863
- Product name
- Mouse Monoclonal hnRNP M Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. NB200-315 is specific for hnRNP M3 and M4 proteins.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Transcription-dependent nucleocytoplasmic distribution of hnRNP A1 protein in early mouse embryos.
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
Vautier D, Chesné P, Cunha C, Calado A, Renard JP, Carmo-Fonseca M
Journal of cell science 2001 Apr;114(Pt 8):1521-31
Journal of cell science 2001 Apr;114(Pt 8):1521-31
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
Datar KV, Dreyfuss G, Swanson MS
Nucleic acids research 1993 Feb 11;21(3):439-46
Nucleic acids research 1993 Feb 11;21(3):439-46
The human hnRNP M proteins: identification of a methionine/arginine-rich repeat motif in ribonucleoproteins.
Datar KV, Dreyfuss G, Swanson MS
Nucleic acids research 1993 Feb 11;21(3):439-46
Nucleic acids research 1993 Feb 11;21(3):439-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: hnRNP M Antibody (2A6) [NB200-315] - Simple Western lane view shows a specific band for hnRNP M in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: hnRNP M Antibody (2A6) [NB200-315] - Analysis of hnRNP M3-M4 in HeLa cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: hnRNP M Antibody (2A6) [NB200-315] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using hnRNP M (2A6) antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear riboprotein staining was observed. Staining was performed by Histowiz.
