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LearnMA1-16674
antibody from Invitrogen Antibodies
Targeting: HNRNPM
CEAR, HNRNPM4, HNRPM, HNRPM4, HTGR1, NAGR1
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- MA1-16674 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hnRNP M3-M4 Monoclonal Antibody (2A6)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for HNRNPM (transient overexpression lysate).
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2A6
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP M3-M4 in HeLa cell lysate using Product # MA1-16674.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP M3-M4 in HeLa cell lysate. Sample was incubated in hnRNP M3-M4 monoclonal antibody (Product # MA1-16674).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of hnRNP M3-M4 in 0.05 mg/mL HeLa lysate. Samples were incubated in hnRNP M3-M4 monoclonal antibody (Product # MA1-16674). This experiment was performed under reducing conditions using the 12-230 kDa separation system..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of hnRNP M3-M4 in formalin fixed paraffin-embedded (FFPE) human tonsil. Samples were incubated in hnRNP M3-M4 monoclonal antibody (Product # MA1-16674) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear riboprotein staining was observed. Staining was performed by Histowiz.
