NB200-316

Xiao Q, Ford AL, Xu J, Yan P, Lee KY, Gonzales E, West T, Holtzman DM, Lee JM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2012 Sep 26;32(39):13587-96

CUGBP1 overexpression in mouse skeletal muscle reproduces features of myotonic dystrophy type 1.

Ward AJ, Rimer M, Killian JM, Dowling JJ, Cooper TA
Human molecular genetics 2010 Sep 15;19(18):3614-22

Ectopic expression of CGG containing mRNA is neurotoxic in mammals.

Hashem V, Galloway JN, Mori M, Willemsen R, Oostra BA, Paylor R, Nelson DL
Human molecular genetics 2009 Jul 1;18(13):2443-51

Expanded CTG repeats within the DMPK 3' UTR causes severe skeletal muscle wasting in an inducible mouse model for myotonic dystrophy.

Orengo JP, Chambon P, Metzger D, Mosier DR, Snipes GJ, Cooper TA
Proceedings of the National Academy of Sciences of the United States of America 2008 Feb 19;105(7):2646-51

Inactivation of CUG-BP1/CELF1 causes growth, viability, and spermatogenesis defects in mice.

Kress C, Gautier-Courteille C, Osborne HB, Babinet C, Paillard L
Molecular and cellular biology 2007 Feb;27(3):1146-57

Failure of MBNL1-dependent post-natal splicing transitions in myotonic dystrophy.

Lin X, Miller JW, Mankodi A, Kanadia RN, Yuan Y, Moxley RT, Swanson MS, Thornton CA
Human molecular genetics 2006 Jul 1;15(13):2087-97

Nuclear RNA foci in the heart in myotonic dystrophy.

Mankodi A, Lin X, Blaxall BC, Swanson MS, Thornton CA
Circulation research 2005 Nov 25;97(11):1152-5

Ribonuclear inclusions in skeletal muscle in myotonic dystrophy types 1 and 2.

Mankodi A, Teng-Umnuay P, Krym M, Henderson D, Swanson M, Thornton CA
Annals of neurology 2003 Dec;54(6):760-8

Aberrant regulation of insulin receptor alternative splicing is associated with insulin resistance in myotonic dystrophy.

Savkur RS, Philips AV, Cooper TA
Nature genetics 2001 Sep;29(1):40-7

A family of human RNA-binding proteins related to the Drosophila Bruno translational regulator.

Good PJ, Chen Q, Warner SJ, Herring DC
The Journal of biological chemistry 2000 Sep 15;275(37):28583-92

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.