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LearnMAB9388-100
antibody from R&D Systems
Targeting: CELF1
BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, hNab50, NAB50, NAPOR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB9388-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat CUGBP1/CELF1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CUGBP1/CELF1 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q92879- Isotype
- IgG
- Antibody clone number
- 850717
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat CUGBP1/CELF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat CUGBP1/ CELF1 Monoclonal Antibody (Catalog # MAB9388) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CUGBP1/CELF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CUGBP1/CELF1 in Human Skeletal Muscle. CUGBP1/CELF1 was detected in immersion fixed paraffin-embedded sections of human skeletal muscle using Mouse Anti-Human/Mouse/Rat CUGBP1/CELF1 Monoclonal Antibody (Catalog # MAB9388) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
