AP6340D

antibody from Abgent

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6340D

Provider

Abgent

Proper citation

Abgent Cat#AP6340d, RRID:AB_637018

Product name

Glypican 3 (GPC3) Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

1.4 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

GPC3 Antibody?P544? (cat# AP6340d) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

GPC3 Antibody (P544) (cat# AP6340d) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Immunofluorecence staining of anti-GPC3 Pab (cat# AP6340d) on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine."

Primary Ab dilution

1:10~50