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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX130732 - Provider product page
- Provider
- GeneTex
- Product name
- Glypican-3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GPC3 antibody (GTX130732) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican-3 antibody (GTX130732) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Glypican-3 antibody detects Glypican-3 protein at cell membrane by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Glypican-3 protein stained by Glypican-3 antibody (GTX130732) diluted at 1:400.Blue: Hoechst 33342 staining.
