Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- PAB4434 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4434, RRID:AB_1575253
- Product name
- GPC3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GPC3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of glypican-3 as a novel tumor marker for melanoma.
Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.
Methylation analysis of the glypican 3 gene in embryonal tumours.
Nakatsura T, Kageshita T, Ito S, Wakamatsu K, Monji M, Ikuta Y, Senju S, Ono T, Nishimura Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Oct 1;10(19):6612-21
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Oct 1;10(19):6612-21
Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.
Wichert A, Stege A, Midorikawa Y, Holm PS, Lage H
Oncogene 2004 Jan 29;23(4):945-55
Oncogene 2004 Jan 29;23(4):945-55
Methylation analysis of the glypican 3 gene in embryonal tumours.
Boily G, Saikali Z, Sinnett D
British journal of cancer 2004 Apr 19;90(8):1606-11
British journal of cancer 2004 Apr 19;90(8):1606-11
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorecence staining of GPC3 polyclonal antibody (Cat # PAB4434) on HepG2 cells . The cells were acetone fixated . Antibody dilution of 1 : 50 . Original magnification 1 : 400 . Data and protocol courtesy of Dr . Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine .
- Validation comment
- Immunofluorescence