PAB4434

antibody from Abnova Corporation

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB4434

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB4434, RRID:AB_1575253

Product name

GPC3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of GPC3.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of glypican-3 as a novel tumor marker for melanoma.

Nakatsura T, Kageshita T, Ito S, Wakamatsu K, Monji M, Ikuta Y, Senju S, Ono T, Nishimura Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Oct 1;10(19):6612-21

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Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.

Wichert A, Stege A, Midorikawa Y, Holm PS, Lage H
Oncogene 2004 Jan 29;23(4):945-55

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Methylation analysis of the glypican 3 gene in embryonal tumours.

Boily G, Saikali Z, Sinnett D
British journal of cancer 2004 Apr 19;90(8):1606-11

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of mouse stomach tissue lysate (35 ug/lane) with GPC3 polyclonal antibody (Cat # PAB4434).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorecence staining of GPC3 polyclonal antibody (Cat # PAB4434) on HepG2 cells . The cells were acetone fixated . Antibody dilution of 1 : 50 . Original magnification 1 : 400 . Data and protocol courtesy of Dr . Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine .

Validation comment

Immunofluorescence