LS-C749002

antibody from LSBio

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C749002

Provider

LSBio

Product name

GPC3 / Glypican 3 Antibody LS-C749002

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using GPC3 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 90s.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using GPC3 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse spleen using GPC3 antibody at dilution of 1:100 (40x lens).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse kidney using GPC3 antibody at dilution of 1:100 (40x lens).