MA5-31552

antibody from Invitrogen Antibodies

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31552

Provider

Invitrogen Antibodies

Product name

Glypican 3 Monoclonal Antibody (GT2473)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Keep as concentrated solution.

Antibody clone number

GT2473

Concentration

0.4 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Glypican 3 in whole cell extract using Glypican 3 monoclonal antibody (Product # MA5-31552) using 30 µg of sample at a dilution of 1:1000. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Glypican 3 was performed by separating 30 µg of untreated (–) and treated (+) HepG2 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552). Untreated (–) and treated (+) HepG2 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) diluted at 1:2,000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Glypican 3 Monoclonal Antibody (GT2473) detects Glypican-3 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glypican-3 protein stained by Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) diluted at 1:250. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Glypican 3 in paraffin-embedded human hepatocellular carcinoma using Glypican 3 monoclonal antibody (Product # MA5-31552) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Glypican 3 Monoclonal Antibody (GT2473) detects Glypican-3 protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated). Sample: Paraffin-embedded human liver cancer. Glypican-3 stained by Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) diluted at 1:100. Antigen Retrieval: Citrate buffer, 20 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Glypican 3 Monoclonal Antibody (GT2473) detects Glypican-3 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human pancreatic cancer. Glypican-3 stained by Glypican 3 Monoclonal Antibody (GT2473) (Product # MA5-31552) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.