V3714

antibody from NSJ Bioreagents

Targeting: GPC3 DGSX, OCI-5, SDYS, SGB, SGBS, SGBS1

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V3714

Provider

NSJ Bioreagents

Product name

Recombinant GPC3 Antibody / Glypican-3

Antibody type

Monoclonal

Description

This highly specific Recombinant GPC3 antibody is suitable for use in Flow cytometry/Immunofluorescence/Immunohistochemistry applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

rGPC3/863

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the recombinant GPC3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of methanol-fixed HepG2 cells with recombinant GPC3 antibody (green, clone rGPC3/863) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human hepatocellular carcinoma with recombinant GPC3 antibody (clone rGPC3/863). Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of methanol-fixed human HepG2 cells with recombinant GPC3 antibody (clone rGPC3/863); Red=isotype control, Blue= recombinant GPC3 antibody.

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using recombinant GPC3 antibody (clone rGPC3/863). These results demonstrate the foremost specificity of the rGPC3/863 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free recombinant GPC3 antibody (clone rGPC3/863) as confirmation of integrity and purity.