Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- ABIN1449428 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Glypican 3 (GPC3) (AA 345-370), (Middle Region) antibody
- Antibody type
- Polyclonal
- Description
- Protein G Column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- AA 345-370, Middle Region
- Vial size
- 0.4 mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Identification of glypican-3 as a novel tumor marker for melanoma.
Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.
Methylation analysis of the glypican 3 gene in embryonal tumours.
Glypican-3 is overexpressed in human hepatocellular carcinoma.
Glypican-3, overexpressed in hepatocellular carcinoma, modulates FGF2 and BMP-7 signaling.
Nakatsura T, Kageshita T, Ito S, Wakamatsu K, Monji M, Ikuta Y, Senju S, Ono T, Nishimura Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Oct 1;10(19):6612-21
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Oct 1;10(19):6612-21
Glypican-3 is involved in cellular protection against mitoxantrone in gastric carcinoma cells.
Wichert A, Stege A, Midorikawa Y, Holm PS, Lage H
Oncogene 2004 Jan 29;23(4):945-55
Oncogene 2004 Jan 29;23(4):945-55
Methylation analysis of the glypican 3 gene in embryonal tumours.
Boily G, Saikali Z, Sinnett D
British journal of cancer 2004 Apr 19;90(8):1606-11
British journal of cancer 2004 Apr 19;90(8):1606-11
Glypican-3 is overexpressed in human hepatocellular carcinoma.
Sung YK, Hwang SY, Park MK, Farooq M, Han IS, Bae HI, Kim JC, Kim M
Cancer science 2003 Mar;94(3):259-62
Cancer science 2003 Mar;94(3):259-62
Glypican-3, overexpressed in hepatocellular carcinoma, modulates FGF2 and BMP-7 signaling.
Midorikawa Y, Ishikawa S, Iwanari H, Imamura T, Sakamoto H, Miyazono K, Kodama T, Makuuchi M, Aburatani H
International journal of cancer 2003 Feb 10;103(4):455-65
International journal of cancer 2003 Feb 10;103(4):455-65
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- WB
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorecence staining of GPC3 Antibody Cat.-No AP13408PU-N on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1/50. Original magnification 1/400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.