Antibody data
- Antibody Data
- Antigen structure
- References [83]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Flow cytometry [2]
- Other assay [19]
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Validation data
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- Product number
- MA3-914 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PMCA ATPase Monoclonal Antibody (5F10)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA3-914 detects calcium pump of the plasma membrane (PMCA) ATPase from amphibian, canine, chicken, feline, human, mouse, sheep, primate, rabbit, and rat tissues. This antibody detects all four known isoforms of the PMCA ATPase.
- Antibody clone number
- 5F10
- Concentration
- 1 mg/mL
Submitted references Neuroplastin genetically interacts with Cadherin 23 and the encoded isoform Np55 is sufficient for cochlear hair cell function and hearing.
Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells.
Pharmacological modulation of Kv1.3 potassium channel selectively triggers pathological B lymphocyte apoptosis in vivo in a genetic CLL model.
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice.
Plasma Membrane Ca(2+) ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer.
Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome.
The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca(2+)-Dependent Manner.
Increased free Zn(2+) correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn(2+) -transporters in heart failure.
Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.
Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca(2+)-ATPases and Key Regulators of Ca(2+) Clearance.
Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.
Role of STIM1 in the surface expression of SARAF.
Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation.
Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.
Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells.
Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b.
Inhibition of PMCA activity by tau as a function of aging and Alzheimer's neuropathology.
Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice.
Differentially localized acyl-CoA synthetase 4 isoenzymes mediate the metabolic channeling of fatty acids towards phosphatidylinositol.
TRPC6 participates in the regulation of cytosolic basal calcium concentration in murine resting platelets.
Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity.
The membrane potential modulates thrombin-stimulated Ca²⁺ mobilization and platelet aggregation.
Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina.
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis.
Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells.
The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.
Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.
FATP4 contributes as an enzyme to the basal and insulin-mediated fatty acid uptake of C₂C₁₂ muscle cells.
Sodium-calcium exchanger and lipid rafts in pig coronary artery smooth muscle.
Dysregulation of Ca2+ signaling in astrocytes from mice lacking amyloid precursor protein.
Ca2+-activated Cl− currents are dispensable for olfaction.
Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells.
Mutations in PMCA2 and hereditary deafness: a molecular analysis of the pump defect.
Upregulation of Na+/Ca2+ exchanger and TRPC6 contributes to abnormal Ca2+ homeostasis in arterial smooth muscle cells from Milan hypertensive rats.
Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231.
An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1.
Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout.
Caloxin 1b3: a novel plasma membrane Ca(2+)-pump isoform 1 selective inhibitor that increases cytosolic Ca(2+) in endothelial cells.
Na(+)-K(+)-ATPase and Ca(2+) clearance proteins in smooth muscle: a functional unit.
Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells.
Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions.
Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel.
Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle.
Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation.
Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps.
Regulation of plasma membrane Ca2+-ATPase in human platelets by calpain.
Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapses.
Protein stability and the evolution of the cell membrane.
Plasma membrane calcium pumps in mouse olfactory sensory neurons.
Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.
Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in motor learning and memory.
Sarco/endoplasmic reticulum Ca2+ATPase type 3 isoforms (SERCA3b and SERCA3f): distinct roles in cell adhesion and ER stress.
Role of plasma membrane Ca2+-ATPase in contraction-relaxation processes of the bladder: evidence from PMCA gene-ablated mice.
Inhibitory interaction of the 14-3-3{epsilon} protein with isoform 4 of the plasma membrane Ca(2+)-ATPase pump.
Antisense-mediated Inhibition of the plasma membrane calcium-ATPase suppresses proliferation of MCF-7 cells.
Adenovirally delivered shRNA strongly inhibits Na+-Ca2+ exchanger expression but does not prevent contraction of neonatal cardiomyocytes.
Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice.
Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3.
Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4.
Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).
Basic fibroblast growth factor (bFGF) regulation of the plasma membrane calcium ATPase (PMCA) as part of an anti-apoptotic mechanism of action.
Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta.
Cardiac excitation-contraction coupling in the absence of Na(+) - Ca2+ exchange.
Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities.
Localization of cardiac sodium channels in caveolin-rich membrane domains: regulation of sodium current amplitude.
Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells.
nNOS in canine lower esophageal sphincter: colocalized with Cav-1 and Ca2+-handling proteins?
Plasma membrane Ca(2+)-ATPase associates with the cytoskeleton in activated platelets through a PDZ-binding domain.
Localization of sequences within the C-terminal domain of the cystic fibrosis transmembrane conductance regulator which impact maturation and stability.
Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?
Regulation of plasma membrane Ca2+-ATPase by small GTPases and phosphoinositides in human platelets.
Tyrosine phosphorylation of human platelet plasma membrane Ca(2+)-ATPase in hypertension.
Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation.
Molecular dissection of Ca2+ efflux in immortalized proximal tubule cells.
Expression of the plasma membrane Ca2+-ATPase in myogenic cells.
Thapsigargin-sensitive Ca(2+)-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells.
Localization and identification of Ca2+ATPases in highly purified human platelet plasma and intracellular membranes. Evidence that the monoclonal antibody PL/IM 430 recognizes the SERCA 3 Ca2+ATPase in human platelets.
Distribution of plasma membrane Ca(2+)-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes.
Caveolae from luminal plasmalemma of rat lung endothelium: microdomains enriched in caveolin, Ca(2+)-ATPase, and inositol trisphosphate receptor.
Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump.
Newton S, Kong F, Carlton AJ, Aguilar C, Parker A, Codner GF, Teboul L, Wells S, Brown SDM, Marcotti W, Bowl MR
PLoS genetics 2022 Jan;18(1):e1009937
PLoS genetics 2022 Jan;18(1):e1009937
Orai1α, but not Orai1β, co-localizes with TRPC1 and is required for its plasma membrane location and activation in HeLa cells.
Sanchez-Collado J, Lopez JJ, Jardin I, Berna-Erro A, Camello PJ, Cantonero C, Smani T, Salido GM, Rosado JA
Cellular and molecular life sciences : CMLS 2022 Jan 6;79(1):33
Cellular and molecular life sciences : CMLS 2022 Jan 6;79(1):33
Pharmacological modulation of Kv1.3 potassium channel selectively triggers pathological B lymphocyte apoptosis in vivo in a genetic CLL model.
Severin F, Urbani A, Varanita T, Bachmann M, Azzolini M, Martini V, Pizzi M, Tos APD, Frezzato F, Mattarei A, Ghia P, Bertilaccio MTS, Gulbins E, Paradisi C, Zoratti M, Semenzato GC, Leanza L, Trentin L, Szabò I
Journal of experimental & clinical cancer research : CR 2022 Feb 16;41(1):64
Journal of experimental & clinical cancer research : CR 2022 Feb 16;41(1):64
Neuronal Autophagy Regulates Presynaptic Neurotransmission by Controlling the Axonal Endoplasmic Reticulum.
Kuijpers M, Kochlamazashvili G, Stumpf A, Puchkov D, Swaminathan A, Lucht MT, Krause E, Maritzen T, Schmitz D, Haucke V
Neuron 2021 Jan 20;109(2):299-313.e9
Neuron 2021 Jan 20;109(2):299-313.e9
Striatal infusion of cholesterol promotes dose-dependent behavioral benefits and exerts disease-modifying effects in Huntington's disease mice.
Birolini G, Valenza M, Di Paolo E, Vezzoli E, Talpo F, Maniezzi C, Caccia C, Leoni V, Taroni F, Bocchi VD, Conforti P, Sogne E, Petricca L, Cariulo C, Verani M, Caricasole A, Falqui A, Biella G, Cattaneo E
EMBO molecular medicine 2020 Oct 7;12(10):e12519
EMBO molecular medicine 2020 Oct 7;12(10):e12519
Plasma Membrane Ca(2+) ATPase Isoform 4 (PMCA4) Has an Important Role in Numerous Hallmarks of Pancreatic Cancer.
Sritangos P, Pena Alarcon E, James AD, Sultan A, Richardson DA, Bruce JIE
Cancers 2020 Jan 16;12(1)
Cancers 2020 Jan 16;12(1)
Computational and cellular studies reveal structural destabilization and degradation of MLH1 variants in Lynch syndrome.
Abildgaard AB, Stein A, Nielsen SV, Schultz-Knudsen K, Papaleo E, Shrikhande A, Hoffmann ER, Bernstein I, Gerdes AM, Takahashi M, Ishioka C, Lindorff-Larsen K, Hartmann-Petersen R
eLife 2019 Nov 7;8
eLife 2019 Nov 7;8
The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca(2+)-Dependent Manner.
Roest G, Hesemans E, Welkenhuyzen K, Luyten T, Engedal N, Bultynck G, Parys JB
Cells 2018 Nov 30;7(12)
Cells 2018 Nov 30;7(12)
Increased free Zn(2+) correlates induction of sarco(endo)plasmic reticulum stress via altered expression levels of Zn(2+) -transporters in heart failure.
Olgar Y, Durak A, Tuncay E, Bitirim CV, Ozcinar E, Inan MB, Tokcaer-Keskin Z, Akcali KC, Akar AR, Turan B
Journal of cellular and molecular medicine 2018 Mar;22(3):1944-1956
Journal of cellular and molecular medicine 2018 Mar;22(3):1944-1956
Glutamate Deregulation in Ketamine-Induced Psychosis-A Potential Role of PSD95, NMDA Receptor and PMCA Interaction.
Lisek M, Ferenc B, Studzian M, Pulaski L, Guo F, Zylinska L, Boczek T
Frontiers in cellular neuroscience 2017;11:181
Frontiers in cellular neuroscience 2017;11:181
Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca(2+)-ATPases and Key Regulators of Ca(2+) Clearance.
Schmidt N, Kollewe A, Constantin CE, Henrich S, Ritzau-Jost A, Bildl W, Saalbach A, Hallermann S, Kulik A, Fakler B, Schulte U
Neuron 2017 Nov 15;96(4):827-838.e9
Neuron 2017 Nov 15;96(4):827-838.e9
Sphingomyelin-induced inhibition of the plasma membrane calcium ATPase causes neurodegeneration in type A Niemann-Pick disease.
Pérez-Cañamás A, Benvegnù S, Rueda CB, Rábano A, Satrústegui J, Ledesma MD
Molecular psychiatry 2017 May;22(5):711-723
Molecular psychiatry 2017 May;22(5):711-723
Role of STIM1 in the surface expression of SARAF.
Albarran L, Regodón S, Salido GM, Lopez JJ, Rosado JA
Channels (Austin, Tex.) 2017 Jan 2;11(1):84-88
Channels (Austin, Tex.) 2017 Jan 2;11(1):84-88
Increased proliferation of late-born retinal progenitor cells by gestational lead exposure delays rod and bipolar cell differentiation.
Chaney SY, Mukherjee S, Giddabasappa A, Rueda EM, Hamilton WR, Johnson JE Jr, Fox DA
Molecular vision 2016;22:1468-1489
Molecular vision 2016;22:1468-1489
Estrogen Enhances Linkage in the Vascular Endothelial Calmodulin Network via a Feedforward Mechanism at the G Protein-coupled Estrogen Receptor 1.
Tran QK, Firkins R, Giles J, Francis S, Matnishian V, Tran P, VerMeer M, Jasurda J, Burgard MA, Gebert-Oberle B
The Journal of biological chemistry 2016 May 13;291(20):10805-23
The Journal of biological chemistry 2016 May 13;291(20):10805-23
Ca2+ removal by the plasma membrane Ca2+-ATPase influences the contribution of mitochondria to activity-dependent Ca2+ dynamics in Aplysia neuroendocrine cells.
Groten CJ, Rebane JT, Hodgson HM, Chauhan AK, Blohm G, Magoski NS
Journal of neurophysiology 2016 Jun 1;115(5):2615-34
Journal of neurophysiology 2016 Jun 1;115(5):2615-34
Evolutionarily conserved intercalated disc protein Tmem65 regulates cardiac conduction and connexin 43 function.
Sharma P, Abbasi C, Lazic S, Teng ACT, Wang D, Dubois N, Ignatchenko V, Wong V, Liu J, Araki T, Tiburcy M, Ackerley C, Zimmermann WH, Hamilton R, Sun Y, Liu PP, Keller G, Stagljar I, Scott IC, Kislinger T, Gramolini AO
Nature communications 2015 Sep 25;6:8391
Nature communications 2015 Sep 25;6:8391
Hetero-oligomeric Complex between the G Protein-coupled Estrogen Receptor 1 and the Plasma Membrane Ca2+-ATPase 4b.
Tran QK, VerMeer M, Burgard MA, Hassan AB, Giles J
The Journal of biological chemistry 2015 May 22;290(21):13293-307
The Journal of biological chemistry 2015 May 22;290(21):13293-307
Inhibition of PMCA activity by tau as a function of aging and Alzheimer's neuropathology.
Berrocal M, Corbacho I, Vázquez-Hernández M, Ávila J, Sepúlveda MR, Mata AM
Biochimica et biophysica acta 2015 Jul;1852(7):1465-76
Biochimica et biophysica acta 2015 Jul;1852(7):1465-76
Cholesterol-loaded nanoparticles ameliorate synaptic and cognitive function in Huntington's disease mice.
Valenza M, Chen JY, Di Paolo E, Ruozi B, Belletti D, Ferrari Bardile C, Leoni V, Caccia C, Brilli E, Di Donato S, Boido MM, Vercelli A, Vandelli MA, Forni F, Cepeda C, Levine MS, Tosi G, Cattaneo E
EMBO molecular medicine 2015 Dec;7(12):1547-64
EMBO molecular medicine 2015 Dec;7(12):1547-64
Differentially localized acyl-CoA synthetase 4 isoenzymes mediate the metabolic channeling of fatty acids towards phosphatidylinositol.
Küch EM, Vellaramkalayil R, Zhang I, Lehnen D, Brügger B, Sreemmel W, Ehehalt R, Poppelreuther M, Füllekrug J
Biochimica et biophysica acta 2014 Feb;1841(2):227-39
Biochimica et biophysica acta 2014 Feb;1841(2):227-39
TRPC6 participates in the regulation of cytosolic basal calcium concentration in murine resting platelets.
Albarran L, Berna-Erro A, Dionisio N, Redondo PC, Lopez E, Lopez JJ, Salido GM, Brull Sabate JM, Rosado JA
Biochimica et biophysica acta 2014 Apr;1843(4):789-96
Biochimica et biophysica acta 2014 Apr;1843(4):789-96
Complete atrial-specific knockout of sodium-calcium exchange eliminates sinoatrial node pacemaker activity.
Groenke S, Larson ED, Alber S, Zhang R, Lamp ST, Ren X, Nakano H, Jordan MC, Karagueuzian HS, Roos KP, Nakano A, Proenza C, Philipson KD, Goldhaber JI
PloS one 2013;8(11):e81633
PloS one 2013;8(11):e81633
The membrane potential modulates thrombin-stimulated Ca²⁺ mobilization and platelet aggregation.
Albarrán L, Dionisio N, López E, Salido GM, Rosado JA
Archives of biochemistry and biophysics 2013 Oct 15;538(2):130-7
Archives of biochemistry and biophysics 2013 Oct 15;538(2):130-7
Targeted expression of anoctamin calcium-activated chloride channels in rod photoreceptor terminals of the rodent retina.
Dauner K, Möbus C, Frings S, Möhrlen F
Investigative ophthalmology & visual science 2013 May 3;54(5):3126-36
Investigative ophthalmology & visual science 2013 May 3;54(5):3126-36
Induction of apoptosis in macrophages via Kv1.3 and Kv1.5 potassium channels.
Leanza L, Zoratti M, Gulbins E, Szabò I
Current medicinal chemistry 2012;19(31):5394-404
Current medicinal chemistry 2012;19(31):5394-404
Mutation of plasma membrane Ca2+ ATPase isoform 3 in a family with X-linked congenital cerebellar ataxia impairs Ca2+ homeostasis.
Zanni G, Calì T, Kalscheuer VM, Ottolini D, Barresi S, Lebrun N, Montecchi-Palazzi L, Hu H, Chelly J, Bertini E, Brini M, Carafoli E
Proceedings of the National Academy of Sciences of the United States of America 2012 Sep 4;109(36):14514-9
Proceedings of the National Academy of Sciences of the United States of America 2012 Sep 4;109(36):14514-9
Inhibitors of mitochondrial Kv1.3 channels induce Bax/Bak-independent death of cancer cells.
Leanza L, Henry B, Sassi N, Zoratti M, Chandy KG, Gulbins E, Szabò I
EMBO molecular medicine 2012 Jul;4(7):577-93
EMBO molecular medicine 2012 Jul;4(7):577-93
The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.
Chen Q, Mahendrasingam S, Tickle JA, Hackney CM, Furness DN, Fettiplace R
The European journal of neuroscience 2012 Aug;36(3):2302-10
The European journal of neuroscience 2012 Aug;36(3):2302-10
Distinct regulation of cytoplasmic calcium signals and cell death pathways by different plasma membrane calcium ATPase isoforms in MDA-MB-231 breast cancer cells.
Curry MC, Luk NA, Kenny PA, Roberts-Thomson SJ, Monteith GR
The Journal of biological chemistry 2012 Aug 17;287(34):28598-608
The Journal of biological chemistry 2012 Aug 17;287(34):28598-608
FATP4 contributes as an enzyme to the basal and insulin-mediated fatty acid uptake of C₂C₁₂ muscle cells.
Digel M, Staffer S, Ehehalt F, Stremmel W, Ehehalt R, Füllekrug J
American journal of physiology. Endocrinology and metabolism 2011 Nov;301(5):E785-96
American journal of physiology. Endocrinology and metabolism 2011 Nov;301(5):E785-96
Sodium-calcium exchanger and lipid rafts in pig coronary artery smooth muscle.
Kuszczak I, Samson SE, Pande J, Shen DQ, Grover AK
Biochimica et biophysica acta 2011 Mar;1808(3):589-96
Biochimica et biophysica acta 2011 Mar;1808(3):589-96
Dysregulation of Ca2+ signaling in astrocytes from mice lacking amyloid precursor protein.
Linde CI, Baryshnikov SG, Mazzocco-Spezzia A, Golovina VA
American journal of physiology. Cell physiology 2011 Jun;300(6):C1502-12
American journal of physiology. Cell physiology 2011 Jun;300(6):C1502-12
Ca2+-activated Cl− currents are dispensable for olfaction.
Billig GM, Pál B, Fidzinski P, Jentsch TJ
Nature neuroscience 2011 Jun;14(6):763-9
Nature neuroscience 2011 Jun;14(6):763-9
Effect of protein S-glutathionylation on Ca2+ homeostasis in cultured aortic endothelial cells.
Lock JT, Sinkins WG, Schilling WP
American journal of physiology. Heart and circulatory physiology 2011 Feb;300(2):H493-506
American journal of physiology. Heart and circulatory physiology 2011 Feb;300(2):H493-506
Mutations in PMCA2 and hereditary deafness: a molecular analysis of the pump defect.
Giacomello M, De Mario A, Lopreiato R, Primerano S, Campeol M, Brini M, Carafoli E
Cell calcium 2011 Dec;50(6):569-76
Cell calcium 2011 Dec;50(6):569-76
Upregulation of Na+/Ca2+ exchanger and TRPC6 contributes to abnormal Ca2+ homeostasis in arterial smooth muscle cells from Milan hypertensive rats.
Zulian A, Baryshnikov SG, Linde CI, Hamlyn JM, Ferrari P, Golovina VA
American journal of physiology. Heart and circulatory physiology 2010 Sep;299(3):H624-33
American journal of physiology. Heart and circulatory physiology 2010 Sep;299(3):H624-33
Golgi calcium pump secretory pathway calcium ATPase 1 (SPCA1) is a key regulator of insulin-like growth factor receptor (IGF1R) processing in the basal-like breast cancer cell line MDA-MB-231.
Grice DM, Vetter I, Faddy HM, Kenny PA, Roberts-Thomson SJ, Monteith GR
The Journal of biological chemistry 2010 Nov 26;285(48):37458-66
The Journal of biological chemistry 2010 Nov 26;285(48):37458-66
An investigation of the occurrence and properties of the mitochondrial intermediate-conductance Ca2+-activated K+ channel mtKCa3.1.
Sassi N, De Marchi U, Fioretti B, Biasutto L, Gulbins E, Franciolini F, Szabò I, Zoratti M
Biochimica et biophysica acta 2010 Jun-Jul;1797(6-7):1260-7
Biochimica et biophysica acta 2010 Jun-Jul;1797(6-7):1260-7
Sodium accumulation promotes diastolic dysfunction in end-stage heart failure following Serca2 knockout.
Louch WE, Hougen K, Mørk HK, Swift F, Aronsen JM, Sjaastad I, Reims HM, Roald B, Andersson KB, Christensen G, Sejersted OM
The Journal of physiology 2010 Feb 1;588(Pt 3):465-78
The Journal of physiology 2010 Feb 1;588(Pt 3):465-78
Caloxin 1b3: a novel plasma membrane Ca(2+)-pump isoform 1 selective inhibitor that increases cytosolic Ca(2+) in endothelial cells.
Szewczyk MM, Pande J, Akolkar G, Grover AK
Cell calcium 2010 Dec;48(6):352-7
Cell calcium 2010 Dec;48(6):352-7
Na(+)-K(+)-ATPase and Ca(2+) clearance proteins in smooth muscle: a functional unit.
Pritchard TJ, Bowman PS, Jefferson A, Tosun M, Lynch RM, Paul RJ
American journal of physiology. Heart and circulatory physiology 2010 Aug;299(2):H548-56
American journal of physiology. Heart and circulatory physiology 2010 Aug;299(2):H548-56
Intermediate conductance Ca2+-activated potassium channel (KCa3.1) in the inner mitochondrial membrane of human colon cancer cells.
De Marchi U, Sassi N, Fioretti B, Catacuzzeno L, Cereghetti GM, Szabò I, Zoratti M
Cell calcium 2009 May;45(5):509-16
Cell calcium 2009 May;45(5):509-16
Muscarinic-induced recruitment of plasma membrane Ca2+-ATPase involves PSD-95/Dlg/Zo-1-mediated interactions.
Kruger WA, Yun CC, Monteith GR, Poronnik P
The Journal of biological chemistry 2009 Jan 16;284(3):1820-30
The Journal of biological chemistry 2009 Jan 16;284(3):1820-30
Maitotoxin converts the plasmalemmal Ca(2+) pump into a Ca(2+)-permeable nonselective cation channel.
Sinkins WG, Estacion M, Prasad V, Goel M, Shull GE, Kunze DL, Schilling WP
American journal of physiology. Cell physiology 2009 Dec;297(6):C1533-43
American journal of physiology. Cell physiology 2009 Dec;297(6):C1533-43
Reduced expression of sarcalumenin and related Ca2+ -regulatory proteins in aged rat skeletal muscle.
O'Connell K, Gannon J, Doran P, Ohlendieck K
Experimental gerontology 2008 Oct;43(10):958-61
Experimental gerontology 2008 Oct;43(10):958-61
Internalization of plasma membrane Ca2+-ATPase during Xenopus oocyte maturation.
El-Jouni W, Haun S, Machaca K
Developmental biology 2008 Dec 1;324(1):99-107
Developmental biology 2008 Dec 1;324(1):99-107
Functional and immunocytochemical evidence for the expression and localization of the secretory pathway Ca2+-ATPase isoform 1 (SPCA1) in cerebellum relative to other Ca2+ pumps.
Sepúlveda MR, Berrocal M, Marcos D, Wuytack F, Mata AM
Journal of neurochemistry 2007 Nov;103(3):1009-18
Journal of neurochemistry 2007 Nov;103(3):1009-18
Regulation of plasma membrane Ca2+-ATPase in human platelets by calpain.
Brown CS, Dean WL
Platelets 2007 May;18(3):207-11
Platelets 2007 May;18(3):207-11
Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapses.
Johnson JE Jr, Perkins GA, Giddabasappa A, Chaney S, Xiao W, White AD, Brown JM, Waggoner J, Ellisman MH, Fox DA
Molecular vision 2007 Jun 15;13:887-919
Molecular vision 2007 Jun 15;13:887-919
Protein stability and the evolution of the cell membrane.
Mas-Oliva J, Delgado-Coello B
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2007 Jul-Aug;146(1-2):207-213
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2007 Jul-Aug;146(1-2):207-213
Plasma membrane calcium pumps in mouse olfactory sensory neurons.
Weeraratne SD, Valentine M, Cusick M, Delay R, Van Houten JL
Chemical senses 2006 Oct;31(8):725-30
Chemical senses 2006 Oct;31(8):725-30
Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.
Nurden P, Debili N, Vainchenker W, Bobe R, Bredoux R, Corvazier E, Combrie R, Fressinaud E, Meyer D, Nurden AT, Enouf J
Blood 2006 Oct 15;108(8):2587-95
Blood 2006 Oct 15;108(8):2587-95
Importance of K+-dependent Na+/Ca2+-exchanger 2, NCKX2, in motor learning and memory.
Li XF, Kiedrowski L, Tremblay F, Fernandez FR, Perizzolo M, Winkfein RJ, Turner RW, Bains JS, Rancourt DE, Lytton J
The Journal of biological chemistry 2006 Mar 10;281(10):6273-82
The Journal of biological chemistry 2006 Mar 10;281(10):6273-82
Sarco/endoplasmic reticulum Ca2+ATPase type 3 isoforms (SERCA3b and SERCA3f): distinct roles in cell adhesion and ER stress.
Chaâbane C, Corvazier E, Bredoux R, Dally S, Raïes A, Villemain A, Dupuy E, Enouf J, Bobe R
Biochemical and biophysical research communications 2006 Jul 14;345(4):1377-85
Biochemical and biophysical research communications 2006 Jul 14;345(4):1377-85
Role of plasma membrane Ca2+-ATPase in contraction-relaxation processes of the bladder: evidence from PMCA gene-ablated mice.
Liu L, Ishida Y, Okunade G, Shull GE, Paul RJ
American journal of physiology. Cell physiology 2006 Apr;290(4):C1239-47
American journal of physiology. Cell physiology 2006 Apr;290(4):C1239-47
Inhibitory interaction of the 14-3-3{epsilon} protein with isoform 4 of the plasma membrane Ca(2+)-ATPase pump.
Rimessi A, Coletto L, Pinton P, Rizzuto R, Brini M, Carafoli E
The Journal of biological chemistry 2005 Nov 4;280(44):37195-203
The Journal of biological chemistry 2005 Nov 4;280(44):37195-203
Antisense-mediated Inhibition of the plasma membrane calcium-ATPase suppresses proliferation of MCF-7 cells.
Lee WJ, Robinson JA, Holman NA, McCall MN, Roberts-Thomson SJ, Monteith GR
The Journal of biological chemistry 2005 Jul 22;280(29):27076-84
The Journal of biological chemistry 2005 Jul 22;280(29):27076-84
Adenovirally delivered shRNA strongly inhibits Na+-Ca2+ exchanger expression but does not prevent contraction of neonatal cardiomyocytes.
Hurtado C, Ander BP, Maddaford TG, Lukas A, Hryshko LV, Pierce GN
Journal of molecular and cellular cardiology 2005 Apr;38(4):647-54
Journal of molecular and cellular cardiology 2005 Apr;38(4):647-54
Aberrant localization of intracellular organelles, Ca2+ signaling, and exocytosis in Mist1 null mice.
Luo X, Shin DM, Wang X, Konieczny SF, Muallem S
The Journal of biological chemistry 2005 Apr 1;280(13):12668-75
The Journal of biological chemistry 2005 Apr 1;280(13):12668-75
Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3.
Malmberg EK, Andersson CX, Gentzsch M, Chen JH, Mengos A, Cui L, Hansson GC, Riordan JR
Journal of cell science 2004 Nov 1;117(Pt 23):5535-41
Journal of cell science 2004 Nov 1;117(Pt 23):5535-41
Targeted ablation of plasma membrane Ca2+-ATPase (PMCA) 1 and 4 indicates a major housekeeping function for PMCA1 and a critical role in hyperactivated sperm motility and male fertility for PMCA4.
Okunade GW, Miller ML, Pyne GJ, Sutliff RL, O'Connor KT, Neumann JC, Andringa A, Miller DA, Prasad V, Doetschman T, Paul RJ, Shull GE
The Journal of biological chemistry 2004 Aug 6;279(32):33742-50
The Journal of biological chemistry 2004 Aug 6;279(32):33742-50
Expression of calcium transporters in the retina of the tiger salamander (Ambystoma tigrinum).
Krizaj D, Liu X, Copenhagen DR
The Journal of comparative neurology 2004 Aug 2;475(4):463-80
The Journal of comparative neurology 2004 Aug 2;475(4):463-80
Basic fibroblast growth factor (bFGF) regulation of the plasma membrane calcium ATPase (PMCA) as part of an anti-apoptotic mechanism of action.
Peluso JJ
Biochemical pharmacology 2003 Oct 15;66(8):1363-9
Biochemical pharmacology 2003 Oct 15;66(8):1363-9
Localization of alkaline phosphatase and Ca2+-ATPase in the cat placenta.
Champion EE, Glazier JD, Greenwood SL, Mann SJ, Rawlings JM, Sibley CP, Jones CJ
Placenta 2003 May;24(5):453-61
Placenta 2003 May;24(5):453-61
Cardiac excitation-contraction coupling in the absence of Na(+) - Ca2+ exchange.
Reuter H, Henderson SA, Han T, Mottino GA, Frank JS, Ross RS, Goldhaber JI, Philipson KD
Cell calcium 2003 Jul;34(1):19-26
Cell calcium 2003 Jul;34(1):19-26
Homer 2 tunes G protein-coupled receptors stimulus intensity by regulating RGS proteins and PLCbeta GAP activities.
Shin DM, Dehoff M, Luo X, Kang SH, Tu J, Nayak SK, Ross EM, Worley PF, Muallem S
The Journal of cell biology 2003 Jul 21;162(2):293-303
The Journal of cell biology 2003 Jul 21;162(2):293-303
Localization of cardiac sodium channels in caveolin-rich membrane domains: regulation of sodium current amplitude.
Yarbrough TL, Lu T, Lee HC, Shibata EF
Circulation research 2002 Mar 8;90(4):443-9
Circulation research 2002 Mar 8;90(4):443-9
Ca2+-dependent protein kinase--a modulation of the plasma membrane Ca2+-ATPase in parotid acinar cells.
Bruce JI, Yule DI, Shuttleworth TJ
The Journal of biological chemistry 2002 Dec 13;277(50):48172-81
The Journal of biological chemistry 2002 Dec 13;277(50):48172-81
nNOS in canine lower esophageal sphincter: colocalized with Cav-1 and Ca2+-handling proteins?
Daniel EE, Jury J, Wang YF
American journal of physiology. Gastrointestinal and liver physiology 2001 Oct;281(4):G1101-14
American journal of physiology. Gastrointestinal and liver physiology 2001 Oct;281(4):G1101-14
Plasma membrane Ca(2+)-ATPase associates with the cytoskeleton in activated platelets through a PDZ-binding domain.
Zabe M, Dean WL
The Journal of biological chemistry 2001 May 4;276(18):14704-9
The Journal of biological chemistry 2001 May 4;276(18):14704-9
Localization of sequences within the C-terminal domain of the cystic fibrosis transmembrane conductance regulator which impact maturation and stability.
Gentzsch M, Riordan JR
The Journal of biological chemistry 2001 Jan 12;276(2):1291-8
The Journal of biological chemistry 2001 Jan 12;276(2):1291-8
Are B-type Ca2+ channels of cardiac myocytes akin to the passive ion channel in the plasma membrane Ca2+ pump?
Antoine S, Pinet C, Coulombe A
The Journal of membrane biology 2001 Jan 1;179(1):37-50
The Journal of membrane biology 2001 Jan 1;179(1):37-50
Regulation of plasma membrane Ca2+-ATPase by small GTPases and phosphoinositides in human platelets.
Rosado JA, Sage SO
The Journal of biological chemistry 2000 Jun 30;275(26):19529-35
The Journal of biological chemistry 2000 Jun 30;275(26):19529-35
Tyrosine phosphorylation of human platelet plasma membrane Ca(2+)-ATPase in hypertension.
Blankenship KA, Dawson CB, Aronoff GR, Dean WL
Hypertension (Dallas, Tex. : 1979) 2000 Jan;35(1 Pt 1):103-7
Hypertension (Dallas, Tex. : 1979) 2000 Jan;35(1 Pt 1):103-7
Regulation of platelet plasma membrane Ca2+-ATPase by cAMP-dependent and tyrosine phosphorylation.
Dean WL, Chen D, Brandt PC, Vanaman TC
The Journal of biological chemistry 1997 Jun 13;272(24):15113-9
The Journal of biological chemistry 1997 Jun 13;272(24):15113-9
Molecular dissection of Ca2+ efflux in immortalized proximal tubule cells.
White KE, Gesek FA, Nesbitt T, Drezner MK, Friedman PA
The Journal of general physiology 1997 Feb;109(2):217-28
The Journal of general physiology 1997 Feb;109(2):217-28
Expression of the plasma membrane Ca2+-ATPase in myogenic cells.
Hammes A, Oberdorf-Maass S, Jenatschke S, Pelzer T, Maass A, Gollnick F, Meyer R, Afflerbach J, Neyses L
The Journal of biological chemistry 1996 Nov 29;271(48):30816-22
The Journal of biological chemistry 1996 Nov 29;271(48):30816-22
Thapsigargin-sensitive Ca(2+)-ATPases account for Ca2+ uptake to inositol 1,4,5-trisphosphate-sensitive and caffeine-sensitive Ca2+ stores in adrenal chromaffin cells.
Poulsen JC, Caspersen C, Mathiasen D, East JM, Tunwell RE, Lai FA, Maeda N, Mikoshiba K, Treiman M
The Biochemical journal 1995 May 1;307 ( Pt 3)(Pt 3):749-58
The Biochemical journal 1995 May 1;307 ( Pt 3)(Pt 3):749-58
Localization and identification of Ca2+ATPases in highly purified human platelet plasma and intracellular membranes. Evidence that the monoclonal antibody PL/IM 430 recognizes the SERCA 3 Ca2+ATPase in human platelets.
Bokkala S, el-Daher SS, Kakkar VV, Wuytack F, Authi KS
The Biochemical journal 1995 Mar 15;306 ( Pt 3)(Pt 3):837-42
The Biochemical journal 1995 Mar 15;306 ( Pt 3)(Pt 3):837-42
Distribution of plasma membrane Ca(2+)-ATPase and inositol 1,4,5-trisphosphate receptor in human platelet membranes.
Dean WL, Quinton TM
Cell calcium 1995 Jan;17(1):65-70
Cell calcium 1995 Jan;17(1):65-70
Caveolae from luminal plasmalemma of rat lung endothelium: microdomains enriched in caveolin, Ca(2+)-ATPase, and inositol trisphosphate receptor.
Schnitzer JE, Oh P, Jacobson BS, Dvorak AM
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 28;92(5):1759-63
Proceedings of the National Academy of Sciences of the United States of America 1995 Feb 28;92(5):1759-63
Use of expression mutants and monoclonal antibodies to map the erythrocyte Ca2+ pump.
Adamo HP, Caride AJ, Penniston JT
The Journal of biological chemistry 1992 Jul 15;267(20):14244-9
The Journal of biological chemistry 1992 Jul 15;267(20):14244-9
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- Western blot analysis of PMCA ATPase was performed by loading 25 µg of U251 (lane 1), human brain (lane 2) and C2C12 (lane 3) onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a PMCA ATPase monoclonal antibody (Product # MA3-914) at a dilution of 1:2000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~140 kDa.
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- Immunofluorescent analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) (Product # MA3-914) shows staining in C6 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase (Product # MA3-914) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
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- Immunofluorescent analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) (Product # MA3-914) shows staining in Hela Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase (Product # MA3-914) at a dilution of 1:100 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
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- Immunofluorescent analysis of PMCA ATPase using Anti-PMCA ATPase Monoclonal Antibody (5F10) (Product # MA3-914) shows staining in U251 Cells. PMCA ATPase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing PMCA ATPase (Product # MA3-914) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.
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- Immunohistochemistry was performed on normal deparaffinized Human brain tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PMCA ATPase (Product # MA3-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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- Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing PMCA ATPase (Product # MA3-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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- Immunohistochemistry was performed on normal deparaffinized Human tonsil tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing PMCA ATPase (Product # MA3-914) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
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- Flow cytometry analysis of PMCA ATPase in Hela cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PMCA ATPase monoclonal antibody (Product # MA3-914) at a dilution of 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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- Flow cytometry analysis of PMCA ATPase in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a PMCA ATPase monoclonal antibody (Product # MA3-914) at a dilution of 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.
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- Figure 4 Western blot analysis of PMCA protein in synaptosomal membranes. The protein level was determined by immunoblotting (A) using 5F10 antibody recognizing all PMCA isoforms as well as isoform-specific antibodies. The intensity of bands was quantitated by densitometry (B) . The results are presented as AU obtained after normalization to endogenous Na + /K + -ATPase level. Representative blots are shown. Arrows indicate bands taken for quantitative analysis. * P < 0.05 ketamine treated vs. saline, n = 5. CB, cerebellum; H, hippocampus; ST, striatum.
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- Figure 1 Expression of NCX1 and other Ca handling proteins in atrial-specific NCX1 knockout mice. A . Immunoblot (top) of mouse atrial (A) and ventricular (V) homogenate using an antibody to NCX1 in wildtype control (C) and atrial-specific NCX1 KO mice. There is complete absence of NCX1 protein in the immunoblot. The new band in the KO lanes at ~110 kDa represents nonfunctional NCX in KO atria after excision of exon 11 by Cre recombinase [13] . Ventricular expression of NCX1 is unaffected in atrial-specific KO mice. The bottom panel shows immunofluorescence of isolated SAN node myocytes from control (C) and KO hearts. Myocytes were co-immunolabled with antibodies against HCN4 and NCX1. Both control and KO SAN cells stained positive for HCN4, but only control SAN cells showed staining of NCX at the membrane. B . Immunoblots of sarcoendoplasmic reticulum Ca ATPase (SERCA), plasma membrane Ca pump (PMCA; the lower band at 72 kDa represents an active proteolytic fragment), and dihydropyridine receptor (DHPR) in control (C) and NCX1 KO atria. Note the reduction in SERCA and the increase in PMCA and DHPR in KO.
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- Figure 4 Western blot analysis of PMCA protein in synaptosomal membranes. The protein level was determined by immunoblotting (A) using 5F10 antibody recognizing all PMCA isoforms as well as isoform-specific antibodies. The intensity of bands was quantitated by densitometry (B) . The results are presented as AU obtained after normalization to endogenous Na + /K + -ATPase level. Representative blots are shown. Arrows indicate bands taken for quantitative analysis. * P < 0.05 ketamine treated vs. saline, n = 5. CB, cerebellum; H, hippocampus; ST, striatum.
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- Figure 7 Lamination patterns and differential compartmentation of pan-plasma membrane Ca 2+ ATPase and Na + -Ca 2+ exchanger isoform 1 in rod spherules and cone pedicles. A : Confocal image of retina immunolabeled for pan-plasma membrane Ca 2+ ATPase (pan-PMCA). The double white arrowheads identify strongly labeled PMCA bands in each IPL sublamina. The scale bar applies to A , D and represents 40 mum. B - D : PMCA preferentially labels rod spherules. OPL double labeled with markers for PMCA (green), vesicular glutamate transporter 1 (VGluT1: blue) and/or M-cone arrestin (M-CAr: red). Scale bar equal 20 mum. B : VGluT1, which labels photoreceptor terminals and M-CAr, which labels cones, colocalize in the OPL. This reveals the large dome-shaped cone pedicles (purple pixels) and some cone axons (white arrowheads). C : This high magnification image shows the horseshoe-like appearance of the PMCA-positive rod spherules. In contrast, the retina double labeled with PMCA and M-CAr shows that cone pedicles stain weakly and diffusely for PMCA, except for a discrete band at the top of the pedicle (white arrowheads: yellow pixels). D : A retina double labeled with PMCA and VGluT1 confirms that PMCA extensively labels rod spherules, but only sparsely labels cone pedicles (white arrowheads). E : PKCalpha-positive rod bipolar cells (red) are pan-PMCA-negative (green), although they are in close apposition around the rod spherules (yellow pixels). The scale bar represents 20 mum. F : Retinal
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- Figure 8 NCX1 localizes to ribbon synaptic units of cone pedicles and mitochondria closely associate with PMCA in photoreceptor terminals. A : NCX1-positive cone pedicles (red) and VGluT1 (blue) colocalize in the proximal ONL (white arrowheads: purple pixels). B : Synaptotagmin 1 (green) labels photoreceptor synaptic vesicles. Small NCX1- and synaptotagmin 1-positive puncta colocalize in rod spherules (white arrows: yellow pixels), while larger colocalized clusters are present in cone pedicles (white arrowheads: yellow-orange pixels). Scale bar equal 20 mum. C : Kinesin KIF3A (green) labels photoreceptor ribbons and docked synaptic vesicles. The kinesin-labeled rod spherules have an arc-shaped appearance and colocalize with diffusely located NCX1 (small yellow puncta). In contrast, cone pedicles have large clusters of double labeled NCX1- and kinesin-positive puncta (white arrows: yellow-orange pixels). Scale bar equal 20 mum. D - F : Mitochondria cluster away from the active zone in cone pedicles. Scale for D and E equal 20 mum and for F equal 10 mum. D : NCX1 (red) and PNA (blue) colocalize in cone pedicles (purple pixels). E : Triple labeling with NCX1, PNA and COX IV (green) reveals that the cone pedicles (white ellipsoids) contain multiple mitochondria that are located away from the ribbon synaptic unit. F : Higher magnification image of the same six pedicles in E reveals the COX IV and NCX1 colabeling (white arrowheads) and the distance of the COX IV-positive mitochondr
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- Figure EV1 Behavioral characterization of wt mice following striatal infusion of the high dose of cholesterol and in vivo exogenous cholesterol localization A Latency to fall (seconds) from an accelerating rotarod at 10 weeks of age (3 weeks after cholesterol infusion) in wt ( N = 11); wt ACSF ( N = 11) and wt chol-high ( N = 7) mice. B-E Global motor activity (B), total distance travelled (C), mean velocity (D), and stereotyped movements (E) in an open field at 11 weeks of age (4 weeks after cholesterol infusion) (wt = 11; wt ACSF = 10; wt chol-high = 7). F Discrimination index (%) in the novel object recognition test of wt, wt ACSF, and wt chol-high mice at 11 weeks of age (4 weeks after cholesterol infusion) (wt = 11; wt ACSF = 10; wt chol-high = 7). DI above zero indicates a preference for the novel object; DI below zero indicates a preference for the familiar object. G, H Cholesterol content in the infused striatum (G) and ipsilateral cortex (H) of wt ACSF ( N = 3), wt chol-low ( N = 5), and wt chol-high ( N = 4) mice at 12 weeks of age after 4-week striatal cholesterol infusion. I-M Representative confocal images showing co-localization of BODIPY-chol (green) and TGN46 (I), calnexin (J), Rab9 (K), PMCA-ATPase (L), and LAMP1 (M) (red) in the striatum of R6/2 mice infused with BODIPY-cholesterol. Scale bars: 5 mum. Data information: The data in (A-H) are shown as scatterplot graphs with means +- standard error. Each dot corresponds to the value obtained from each animal.
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- Figure 2 Expression of PMCA isoforms in multiple pancreatic cell lines. ( A ) Representative Western immunoblot showing the relative protein expression of total/pan-PMCA and PMCA isoform 1-4 in pancreatic cancer (MIA PaCa-2 and PANC-1) and non-malignant pancreatic cells (human pancreatic ductal epithelial (HPDE) and human pancreatic stellate cells (hPSC)). Mouse brain lysate was used as a positive control for PMCA expressions and beta-Actin was used as a protein loading control. ( B ) PMCA4 protein expression in each cell line was quantified from Western blot bands and normalized to beta-Actin housekeeping protein. ( C ) The relative expressions of ATP2B1-4 (PMCA1-4 mRNA) in each cell line were quantified by RT-qPCR. Data are expressed as relative mRNA expression normalized to corresponding S18 rRNA controls (2 -DeltaCtau ). Statistical comparisons were made using the Kruskal-Wallis test with Dunn's multiple comparison test and two-way analysis of variance (ANOVA) with Dunnett's multiple comparison test. Data are expressed as mean +- SEM. (n = 4-5, 4 replicates per treatment condition). * represents statistical significance where p < 0.05.
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- Figure 3--figure supplement 1. Solubility of selected MLH1 variants. Transfected cells, either untreated (-) or treated for 16 hr with 10 muM bortezomib (+BZ) were lysed by sonication and immediately separated into supernatant ( S ) and pellet ( P ) fractions by centrifugation. Western blotting using antibodies to MLH1 was employed to determine the amount of MLH1 in the fractions. Blotting with antibodies to GAPDH and PMCA served as loading controls for the soluble and insoluble fraction, respectively.