15134-1-AP

antibody from Invitrogen Antibodies

Targeting: HDGFL2 Hdgfrp2, HRP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

15134-1-AP

Provider

Invitrogen Antibodies

Product name

HRP 2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: TEPARKPGQ KEKRVRPEEK QQAKPVKVER TRKRSEGFSM DRKVEKKKEP SVEEKLQKLH SEIKFALKVD SPDVKRCLNA LEELGTLQVT SQILQKNTDV VATLKKIRRY KANKDVMEKA AEVYTRLKSR VLGPKIEAVQ KVNKAGMEKE KAEEKLAGEE LAGEEAPQEK AEDKPSTDLS APVNGEATSQ KGESAEDKEH EEGRDSEEGP RCGSSEDLHD VREGPDLDRP GSDRQERERA RGDSEALDEE S (421-670 aa encoded by BC000755)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.44 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 15134-1-AP (HRP 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 15134-1-AP (HRP 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human liver tissue were subjected to SDS PAGE followed by western blot with 15134-1-AP (HRP 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 15134-1-AP (HRP 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 15134-1-AP (HRP 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 15134-1-AP ( HRP 2 antibody at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon tissue slide using 15134-1-AP ( HRP 2 antibody at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-HRP 2 (IP:15134-1-AP, 4ug; Detection:15134-1-AP 1:10000) with HepG2 cells lysate 3400ug.