HPA038610

antibody from Atlas Antibodies

Targeting: PLEKHA7 DKFZp686M22243

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA038610

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA038610, RRID:AB_10671746

Product name

Anti-PLEKHA7

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human PLEKHA7, Gene description: pleckstrin homology domain containing, family A member 7, Alternative Gene Names: DKFZp686M22243, Validated applications: WB, IHC, ICC, Uniprot ID: Q6IQ23, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.2 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Actin-dependent recruitment of AGO2 to the zonula adherens

Bridges M, Nair-Menon J, Risner A, Jimenez D, Daulagala A, Kingsley C, Davis M, Kourtidis A, Yap A
Molecular Biology of the Cell 2023;34(13)

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ECM Substrates Impact RNAi Localization at Adherens Junctions of Colon Epithelial Cells

Daulagala A, Kourtidis A
Cells 2022;11(23):3740

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PLEKHA7, an Apical Adherens Junction Protein, Suppresses Inflammatory Breast Cancer in the Context of High E-Cadherin and p120-Catenin Expression

Pence L, Kourtidis A, Feathers R, Haddad M, Sotiriou S, Decker P, Nassar A, Ocal I, Shah S, Anastasiadis P
International Journal of Molecular Sciences 2021;22(3):1275

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Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer

Nair-Menon J, Daulagala A, Connor D, Rutledge L, Penix T, Bridges M, Wellslager B, Spyropoulos D, Timmers C, Broome A, Kourtidis A
International Journal of Molecular Sciences 2020;21(7):2559

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miR-24 Is Elevated in Ulcerative Colitis Patients and Regulates Intestinal Epithelial Barrier Function

Soroosh A, Rankin C, Polytarchou C, Lokhandwala Z, Patel A, Chang L, Pothoulakis C, Iliopoulos D, Padua D
The American Journal of Pathology 2019;189(9):1763-1774

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Cadherin complexes recruit mRNAs and RISC to regulate epithelial cell signaling

Kourtidis A, Necela B, Lin W, Lu R, Feathers R, Asmann Y, Thompson E, Anastasiadis P
Journal of Cell Biology 2017;216(10):3073-3085

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PLEKHA7 defines an apical junctional complex with cytoskeletal associations and miRNA-mediated growth implications

Kourtidis A, Anastasiadis P
Cell Cycle 2016;15(4):498-505

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Binding between the Junctional Proteins Afadin and PLEKHA7 and Implication in the Formation of Adherens Junction in Epithelial Cells

Kurita S, Yamada T, Rikitsu E, Ikeda W, Takai Y
Journal of Biological Chemistry 2013;288(41):29356-29368

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and PLEKHA7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406299).

Sample type

Human

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleus, cytosol & cell junctions.

Sample type

Human